Separation and detection of angiotensin peptides by Cu(II) complexation and capillary electrophoresis with UV and electrochemical detection.
Separation and detection of angiotensin peptides by Cu(II) complexation and capillary electrophoresis with UV and electrochemical detection.
复制标题
通过 Cu(II) 络合和毛细管电泳以及紫外和电化学检测来分离和检测血管紧张素肽。
DOI：
10.1002/1522-2683(200206)23:11
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Lunte,SusanM
中科院分区：
文献类型：
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作者：
Lacher,NathanA;Garrison,KennethE;Lunte,SusanM
The use of capillary electrophoresis (CE) with on‐capillary Cu(II) complexation for the determination of angiotensin and its metabolites is described. The resulting copper‐peptide complexes can be detected using either UV or electrochemical (EC) detection. Optimal reaction and separation conditions for the angiotensin peptides were first determined using CE with UV detection. With UV detection, the limit of detection (signal‐to noise ratio S/N = 3) for native angiotensin II was 18 νM, while the limit of detection (LOD) obtained for the copper‐angiotensin II complex is 2 νM. CE with EC detection was then evaluated, yielding significantly lower LODs – 2 νMfor native angiotensin II and 200 nMfor the copper‐angiotensin II complex. The addition of copper to the run buffer improved the separation and sensitivity for both CE‐UV and CE‐EC detection. The method was demonstrated by monitoring the conversion of angiotensin I to angiotensin II in plasmaviaangiotensin‐converting enzyme (ACE) and subsequent inhibition of ACE by captopril.
