Biodegradation of Pharmaceutical Residues Investigated by SPE‐GC/ITD‐MS and On‐Line Derivatization
Biodegradation of Pharmaceutical Residues Investigated by SPE‐GC/ITD‐MS and On‐Line Derivatization
复制标题
通过 SPE-GC/ITD-MS 和在线衍生化研究药物残留的生物降解
DOI：
--
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
F. Frimmel
中科院分区：
文献类型：
--
作者：
C. Zwiener;T. Glauner;F. Frimmel
采用固相萃取、在线衍生化和离子捕集-质谱法(ITD-MS)研究了药物残留(环丙沙酸、布洛芬、双氯芬酸)的生物降解性。报道了样品前处理和分析测定一步到位的结果。通过选择分子离子(M+)作为母离子与定义的子离子的碰撞诱导解离，在ITD-MS上进行了MS/MS测量。中试污水厂和生物膜反应器在好氧和缺氧条件下运行，以含有10~50 mg/L溶解有机碳的合成污水和浓度为10μg/L的药物作为模拟系统，环纤酸在所有情况下都表现出持久性。中试污水处理厂和好氧生物膜反应器对双氯芬酸和布洛芬显示出类似的结果，这两种物质都被部分降解。这些结果可以解释这些物质在污水和水环境中的存在。在好氧生物膜反应器中，布洛芬的降解率很高，这归因于生物膜对残留物的适应。根据其质谱图，并与文献数据进行比较，可以鉴定出布洛芬的两种代谢物，即布洛芬。羟基布洛芬和羧基布洛芬。
Solid-phase extraction, on-line derivatization, and measurement by ion trap mass spectrometry (ITD-MS) were used to investigate the biological degradation of pharmaceutical residues (clofibric acid, ibuprofen, diclofenac). The results of the single steps of sample pretreatment and analytical determination are reported. MS/MS measurements were performed on an ITD-MS by selecting collision induced dissociation of the molecular ions (M + ) as parent ions to defined daughter ions. A pilot sewage plant and biofilm reactors operating under oxic and anoxic conditions were run as model systems with synthetic sewage water containing 10 to 50 mg/L dissolved organic carbon (DOC) and pharmaceuticals in concentrations of 10 μg/L. Clofibric acid displayed its persistent character in all cases. The pilot sewage plant and the oxic biofilm reactor showed comparable results for diclofenac and ibuprofen, which both were partly degraded. These results can explain the occurrence of these substances in sewage effluents and in the aquatic environment. A high degree of degradation was found especially for ibuprofen in the oxic biofilm reactor, which was attributed to adaptation of the biofilm to the residue. Two metabolites of ibuprofen could be identified on the basis of their mass spectra and comparison with literature data, viz. hydroxyibuprofen and carboxyibuprofen.