Intracellular reactive oxidative stress, cell proliferation and apoptosis of Schwann cells on carbon nanofibrous substrates
Intracellular reactive oxidative stress, cell proliferation and apoptosis of Schwann cells on carbon nanofibrous substrates
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DOI：
10.1016/j.biomaterials.2013.03.055
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发表时间：
2013-07-01
期刊：
影响因子：
14
通讯作者：
Basu, Bikramjit
中科院分区：
文献类型：
--
作者：
Jain, Shilpee;Webster, Thomas J.;Basu, Bikramjit
尽管在开发用于生物医学应用的碳基材料方面进行了大量研究，但碳的毒性仍然是一个主要问题。为了解决这一问题，并从神经组织工程应用的角度探讨神经细胞的细胞命运过程，采用体外生化分析和流式细胞术分析，对聚丙烯腈（PAN）衍生的连续碳纳米纤维和PAN衍生的碳薄膜的体外细胞相容性进行了定量和定性研究。根据细胞内氧化应激的时间依赖性进化，讨论了非晶碳基质上细胞增殖、细胞代谢活性和细胞凋亡等雪旺细胞命运的实验结果。本研究除了证明电纺碳纳米纤维底物对培养的雪旺细胞具有物理引导作用外，还表明连续碳纳米纤维和无定形碳膜在体外没有细胞毒性，不会显著诱导雪旺细胞凋亡，反而有利于其增殖和生长。(C) 2013 Elsevier Ltd.版权所有。
Despite considerable research to develop carbon based materials for biomedical applications, the toxicity of carbon remains a major concern. In order to address this issue as well as to investigate the cell fate processes of neural cells from the perspective of neural tissue engineering applications, the in vitro cytocompatibility of polyacrylonitrile (PAN) derived continuous carbon nanofibers and PAN derived carbon thin films were investigated both quantitatively and qualitatively using in vitro biochemical assays followed by extensive flow cytometry analysis. The experimental results of Schwann cell fate, i.e. cell proliferation, cell metabolic activity and cell apoptosis on amorphous carbon substrates are discussed in reference to the time dependent evolution of intracellular oxidative stress. Apart from providing evidence that an electrospun carbon nanofibrous substrate can physically guide the cultured Schwann cells, this study suggested that continuous carbon nanofibers and amorphous carbon films are not cytotoxic in vitro and do not significantly induce apoptosis of Schwann cells, but in fact even facilitate their proliferation and growth. (C) 2013 Elsevier Ltd. All rights reserved.