EFFECTS OF EGF, BFGF, NGF AND PDGF(BB) ON CELL PROLIFERATIVE, MIGRATORY AND INVASIVE CAPACITIES OF HUMAN BRAIN-TUMOR BIOPSIES INVITRO
EFFECTS OF EGF, BFGF, NGF AND PDGF(BB) ON CELL PROLIFERATIVE, MIGRATORY AND INVASIVE CAPACITIES OF HUMAN BRAIN-TUMOR BIOPSIES INVITRO
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DOI：
10.1002/ijc.2910530206
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发表时间：
1993-01-21
影响因子：
6.4
通讯作者：
LAERUM, OD
中科院分区：
文献类型：
--
作者：
ENGEBRAATEN, O;BJERKVIG, R;LAERUM, OD
本实验采用人原发性脑胶质瘤直接分化的球状体，研究EGF、bFGF、NGF和PDGF（bb）对胶质瘤细胞增殖、迁移和侵袭胎鼠脑组织的影响。EGF增加了13个胶质母细胞瘤中的10个的肿瘤球体体积，而11个肿瘤中的5个对bFGF有反应。在5个肿瘤中，有2个肿瘤的球体体积增加。在8个肿瘤中，PDGF（bb）对肿瘤球体体积没有影响。BUdR标记指数的增加证实了细胞增殖是刺激球状体中观察到的体积增加的原因。EGF刺激细胞迁移5和碱性成纤维细胞生长因子在3的8个肿瘤研究。5例胶质母细胞瘤中有1例NGF刺激细胞迁移，而3例胶质母细胞瘤中有1例对PDGF有反应（bb）。生长因子对从胶质母细胞瘤活检标本制备的球状体的侵袭的影响也在体外使用胎鼠脑聚集体作为靶组织进行了研究。表皮生长因子刺激7的8个胶质母细胞瘤的研究，而碱性成纤维细胞生长因子刺激这些肿瘤中的2个的侵袭。NGF或PDGF（bb）不增加胶质母细胞瘤组织的侵袭性。我们的结果代表了生长因子对复杂肿瘤细胞群体的净效应。我们的结论是外源性生长因子，特别是EGF，增加细胞增殖以及迁移和体外培养的原发性脑肿瘤活检组织的侵袭能力。
Spheroids initiated directly from human primary gliomas were used to investigate the effects of EGF, bFGF, NGF and PDGF(bb) on cell proliferation, migration and invasion into foetal rat brain tissue. EGF increased tumour spheroid volume in 10 of 13 glioblastomas studied, whereas 5 of 11 tumours responded to bFGF. NGF increased the spheroid volume in 2 of 5 tumours. In 8 tumours, PDGF(bb) had no effect on tumour spheroid volume. An increase in BUdR-labelling indices confirmed that cell proliferation was responsible for the volume increase observed in stimulated spheroids. EGF stimulated cell migration in 5 and bFGF in 3 of 8 tumours studied. NGF stimulated cell migration in 1 of 5 glioblastomas, whereas 1 of 3 glioblastomas responded to PDGF(bb). The effects of growth factors on the invasion of spheroids prepared from the glioblastoma biopsy specimens were also studied in vitro using foetal rat brain aggregates as target tissue. EGF stimulated invasion in 7 of 8 glioblastomas studied, whereas bFGF stimulated invasion in 2 of these tumours. NGF or PDGF(bb) did not increase the invasiveness of the glioblastoma tissue. Our results represent the net effect of the growth factors on a complex tumour-cell population. We conclude that exogenously administered growth factors, EGF in particular, increase the cell proliferation as well as migratory and invasive capacities of cultured primary brain tumour biopsies in vitro.