Maintenance of the large-scale depolarization wave in the embryonic chick brain against deprivation of the rhythm generator.
Maintenance of the large-scale depolarization wave in the embryonic chick brain against deprivation of the rhythm generator.
复制标题
维持胚胎鸡大脑中的大规模去极化波，防止节律发生器的剥夺。
DOI：
10.1016/j.neuroscience.2014.02.014
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发表时间：
2014
期刊：
影响因子：
3.3
通讯作者：
K.
中科院分区：
文献类型：
--
作者：
Momose-Sato;Y. and Sato;K.
发育中的神经系统中广泛相关的自发活动是短暂表达的，并且被认为在神经回路形成中发挥着基本作用。去极化波沿着神经轴传播很长的距离，最大程度地延伸到腰骶索和前脑，是这种自发活动的一个例子。尽管去极化波通常在完整的准备中在脊髓中启动，但在分离的脑干中也检测到自发放电。尽管这表明脑干具有产生自发活动的能力，但由具有更高兴奋性的尾部节律发生器控制，但仍然存在许多问题。脑干活动是否只是作为被动结果出现，或者脑干网络中是否发生任何主动变化来补偿这种活动？如果是后者，这种补偿在不同的发育阶段是否同样发生？孤立的脑干中新的节律发生器在哪里？为了回答这些问题，我们光学分析了在 obex 横断前后从雏鸡脑干检测到的活动的时空模式。结果表明，去极化波保持稳态，其特征是兴奋性和/或波中招募的神经元数量增加。在较年轻的胚胎中，这种波更容易维持。此外，我们证明，即使在完整脑干中不再观察到去极化波的阶段，脑干神经元执行这种主动补偿的能力也没有丧失。
Widely correlated spontaneous activity in the developing nervous system is transiently expressed and is considered to play a fundamental role in neural circuit formation. The depolarization wave, which spreads over a long distance along the neuraxis, maximally extending to the lumbosacral cord and forebrain, is an example of this spontaneous activity. Although the depolarization wave is typically initiated in the spinal cord in intact preparations, spontaneous discharges have also been detected in the isolated brainstem. Although this suggests that the brainstem has the ability to generate spontaneous activity, but is paced by a caudal rhythm generator of higher excitability, a number of questions remains. Does brainstem activity simply appear as a passive consequence, or does any active change occur in the brainstem network to compensate for this activity? If the latter is the case, does this compensation occur equally at different developmental stages? Where is the new rhythm generator in the isolated brainstem? To answer these questions, we optically analyzed spatio-temporal patterns of activity detected from the chick brainstem before and after transection at the obex. The results revealed that the depolarization wave was homeostatically maintained, which was characterized by an increase in excitability and/or the number of neurons recruited to the wave. The wave was more easily maintained in younger embryos. Furthermore, we demonstrated that the ability of brainstem neurons to perform such an active compensation was not lost even at the stage when the depolarization wave was no longer observed in the intact brainstem.