INSITU STUDIES OF THE PRIMARY IMMUNE-RESPONSE TO (4-HYDROXY-3-NITROPHENYL)ACETYL .1. THE ARCHITECTURE AND DYNAMICS OF RESPONDING CELL-POPULATIONS
INSITU STUDIES OF THE PRIMARY IMMUNE-RESPONSE TO (4-HYDROXY-3-NITROPHENYL)ACETYL .1. THE ARCHITECTURE AND DYNAMICS OF RESPONDING CELL-POPULATIONS
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DOI：
10.1084/jem.173.5.1165
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发表时间：
1991-05-01
影响因子：
15.3
通讯作者：
KELSOE, G
中科院分区：
文献类型：
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作者：
JACOB, J;KASSIR, R;KELSOE, G
用（4 - 羟基 - 3 - 硝基苯基）乙酰的免疫原性结合物进行初次免疫后，脾脏中会出现两种在解剖学和表型上不同的抗体形成细胞群。早在免疫后2天，就可在动脉周围淋巴鞘周边观察到抗原结合B细胞的聚集灶。这些聚集灶会扩大，在免疫反应第8天可占据多达1%的脾脏体积。之后，聚集灶变小，到第14天在脾脏中几乎消失。第二个有反应的细胞群，即生发中心B细胞，在第8 - 10天出现，并至少持续到免疫后第16天。单个聚集灶和生发中心代表不同的寡克隆群体，它们在初次免疫反应过程中显然经历了体细胞进化。我们认为，聚集灶可能代表未突变B细胞之间主要为争夺抗原的克隆间竞争区域，而生发中心则是突变的姐妹淋巴细胞之间克隆内竞争的部位。
After primary immunization with an immunogenic conjugate of (4-hydroxy-3-nitrophenyl)acetyl, two anatomically and phenotypically distinct populations of antibody-forming cells arise in the spleen. As early as 2 d after immunization, foci of antigen-binding B cells are observed along the periphery of the periarteriolar lymphoid sheaths. These foci expand, occupying as much as 1% of the splenic volume by day 8 of the response. Later, foci grow smaller and are virtually absent from the spleen by day 14. A second responding population, germinal center B cells, appear on day 8-10 and persist at least until day 16 post-immunization. Individual foci and germinal centers represent discrete pauciclonal populations that apparently undergo somatic evolution in the course of the primary response. We suggest that foci may represent regions of predominantly interclonal competition for antigen among unmutated B cells, while germinal centers are sites of intraclonal clonal competition between mutated sister lymphocytes.