Stretch-induced network reconfiguration of collagen fibres in the human facet capsular ligament.
Stretch-induced network reconfiguration of collagen fibres in the human facet capsular ligament.
复制标题
人体小关节囊韧带中胶原纤维的拉伸诱导网络重构。
DOI：
10.1098/rsif.2015.0883
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Winkelstein,BethA
中科院分区：
文献类型：
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作者：
Zhang,Sijia;Bassett,DanielleS;Winkelstein,BethA
生物材料可以显示细胞对外力反应的复杂空间模式。揭示和预测这些模式在材料失效中的作用需要了解细胞局部生物力学环境中空间分布变化之间的统计依赖性，包括细胞外基质中胶原纤维运动学的改变。在这里，我们开发并应用了网络科学方法的新型扩展，以研究人类颈椎小关节囊韧带（FCL）（慢性颈部疼痛的常见来源）的过度拉伸如何影响胶原纤维的局部重组。我们根据定量偏振光成像测量的纤维排列角度的相似性来定义胶原蛋白排列网络。 We quantify the reorganization of these networks following macroscopic loading by describing the dynamic reconfiguration of network communities, regions of the material that display similar fibre alignment angles.随着时间的推移，群落结构的变化顺利发生，表明纤维对负荷的协调适应。此外，灵活性是网络重新配置的一种衡量标准，可追踪异常重新排列 (AR) 开始时 FCL 机械完整性的损失，以及 AR 显示改变的群落结构的区域。这些发现使用新颖的基于网络的技术来解释异常的胶原纤维重组，这是组织衰竭背后的动态且协调的多变量过程。
Biomaterials can display complex spatial patterns of cellular responses to external forces. Revealing and predicting the role of these patterns in material failure require an understanding of the statistical dependencies between spatially distributed changes in a cell's local biomechanical environment, including altered collagen fibre kinematics in the extracellular matrix. Here, we develop and apply a novel extension of network science methods to investigate how excessive tensile stretch of the human cervical facet capsular ligament (FCL), a common source of chronic neck pain, affects the local reorganization of collagen fibres. We define collagen alignment networks based on similarity in fibre alignment angles measured by quantitative polarized light imaging. We quantify the reorganization of these networks following macroscopic loading by describing the dynamic reconfiguration of network communities, regions of the material that display similar fibre alignment angles. Alterations in community structure occur smoothly over time, indicating coordinated adaptation of fibres to loading. Moreover, flexibility, a measure of network reconfiguration, tracks the loss of FCL's mechanical integrity at the onset of anomalous realignment (AR) and regions of AR display altered community structure. These findings use novel network-based techniques to explain abnormal collagen fibre reorganization, a dynamic and coordinated multivariate process underlying tissue failure.