Sixteen hours of fasting differentially affects hepatic and muscle insulin sensitivity in mice
Sixteen hours of fasting differentially affects hepatic and muscle insulin sensitivity in mice
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DOI：
10.1194/jlr.m400440-jlr200
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发表时间：
2005-03-01
影响因子：
6.5
通讯作者：
Corssmit, EPM
中科院分区：
文献类型：
--
作者：
Heijboer, AC;Donga, E;Corssmit, EPM
禁食容易诱发肝脏脂肪变性。肝脏脂肪变性与肝脏胰岛素抵抗有关。本研究的目的是记录野生型小鼠禁食 16 小时对肝脏和骨骼肌胰岛素敏感性的影响，这与 1) 甘油三酯 (TG) 的组织积累和 2) 代谢相关基因 mRNA 表达的变化有关。在肝脏 TG 含量增加的情况下，禁食 16 小时并没有显示出对葡萄糖产生方面的肝脏胰岛素敏感性的影响。然而，在肌肉中，禁食导致胰岛素敏感性增加，肌肉葡萄糖摄取增加，但肌肉 TG 含量没有变化。在肝脏中，禁食导致促进糖异生和TG合成的基因的mRNA表达增加，但参与糖原分解和脂肪酸合成的基因的mRNA表达减少。在肌肉中，发现促进葡萄糖摄取、脂肪生成和 β-氧化的基因 mRNA 表达增加。总之，16小时禁食虽然会导致肝脏脂肪变性，但不会引起肝脏胰岛素抵抗，而肌肉胰岛素敏感性增加，但肌肉TG含量没有变化。因此，禁食会引起组织特异性胰岛素敏感性的差异变化，而肝脏和肌肉的 TG 含量不太可能参与这些变化。
Fasting readily induces hepatic steatosis. Hepatic steatosis is associated with hepatic insulin resistance. The purpose of the present study was to document the effects of 16 h of fasting in wild-type mice on insulin sensitivity in liver and skeletal muscle in relation to 1) tissue accumulation of triglycerides (TGs) and 2) changes in mRNA expression of metabolically relevant genes. Sixteen hours of fasting did not show an effect on hepatic insulin sensitivity in terms of glucose production in the presence of increased hepatic TG content. In muscle, however, fasting resulted in increased insulin sensitivity, with increased muscle glucose uptake without changes in muscle TG content. In liver, fasting resulted in increased mRNA expression of genes promoting gluconeogenesis and TG synthesis but in decreased mRNA expression of genes involved in glycogenolysis and fatty acid synthesis. In muscle, increased mRNA expression of genes promoting glucose uptake, as well as lipogenesis and beta-oxidation, was found. In conclusion, 16 h of fasting does not induce hepatic insulin resistance, although it causes liver steatosis, whereas muscle insulin sensitivity increases without changes in muscle TG content. Therefore, fasting induces differential changes in tissue-specific insulin sensitivity, and liver and muscle TG contents are unlikely to be involved in these changes.