The characteristics and genome analysis of vB_ApiP_XC38, a novel phage infecting Acinetobacter pittii
The characteristics and genome analysis of vB_ApiP_XC38, a novel phage infecting Acinetobacter pittii
复制标题
感染皮蒂不动杆菌的新型噬菌体vB_ApiP_XC38的特性及基因组分析
DOI：
10.1007/s11262-020-01766-0
复制
发表时间：
2020-05-25
期刊：
影响因子：
1.6
通讯作者：
Gu, Jingmin
中科院分区：
文献类型：
--
作者：
Cheng, Mengjun;Luo, Man;Gu, Jingmin
Acinetobacter pittii is an important pathogen causing nosocomial infection worldwide. In this study, a multidrug-resistant A. pittii ABC38 was used as host bacterium to isolate the lytic phage vB_ApiP_XC38. The biological characteristics of vB_ApiP_XC38 were studied and the genome was sequenced and analyzed. vB_ApiP_XC38 belonged to Podoviridae family. The phage had double-stranded genome, which comprised 79,328 bp with 39.58% G+C content displaying very low similarity (< 1% identity) with published genomes of other phages and bacteria. A total of 97 open reading frames (ORFs) were predicted and contained nucleotide metabolism and replication module, structural components module, and lysis module. The ANI, AAI, and phylogenetic analysis indicated that all phages were found distant from vB_ApiP_XC38. Altogether, morphological, genomics, and phylogenetic analysis suggest that vB_ApiP_XC38 is more likely a novel phage of A. pittii.
