A FUNCTIONAL RIBONUCLEOPROTEIN COMPLEX FORMS AROUND THE 5' END OF POLIOVIRUS RNA
A FUNCTIONAL RIBONUCLEOPROTEIN COMPLEX FORMS AROUND THE 5' END OF POLIOVIRUS RNA
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DOI：
10.1016/0092-8674(90)90170-j
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发表时间：
1990-10-19
期刊：
影响因子：
64.5
通讯作者：
BALTIMORE, D
中科院分区：
文献类型：
--
作者：
ANDINO, R;RIECKHOF, GE;BALTIMORE, D
计算机预测的脊髓灰质炎病毒RNA 5‘端的前100个核苷酸存在一个三叶草结构，通过定点突变以及化学和RNA酶探测得到证实。改变正链中的三叶草而不是负链中的三叶草的突变对病毒是致命的。这种RNA三叶草结构将细胞蛋白与病毒蛋白3PCpro和3Dpol结合在一起。RNA三叶草特定区域的突变阻止了这种结合。破坏核糖核蛋白复合体形成的3CPro或RNA突变抑制了病毒的生长，并选择性地影响正链RNA的积累。这些突变的表型逆转恢复了形成复合体的能力。因此，脊髓灰质炎病毒RNA中的三叶草结构在组织参与正链生产的病毒和细胞蛋白方面发挥着核心作用。
The existence of a computer-predicted cloverleaf structure for the first 100 nucleotides at the 5'' end of poliovirus RNA was verified by site-directed mutagenesis and by chemical and RNAase probing. Mutations that modified the cloverleaf in the positive strand but not the negative strand were lethal to the virus. This RNA cloverleaf structure binds a cellular protein and the viral proteins 3PCpro and 3Dpol. Mutations in specific regions of the RNA cloverleaf prevented this binding. Mutations in either 3Cpro or the RNA that disrupted ribonucleoprotein complex formation inhibited virus growth and selectively affected positive strand RNA accumulation. Phenotypic reversion of these mutations restored the ability to form the complex. Thus, a cloverleaf structure in poliovirus RNA plays a central role in organizing viral and cellular proteins involved in positive strand production.