okra and spindle-B encode components of the RAD52 DNA repair pathway and affect meiosis and patterning in Drosophila oogenesis
okra and spindle-B encode components of the RAD52 DNA repair pathway and affect meiosis and patterning in Drosophila oogenesis
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DOI：
10.1101/gad.12.17.2711
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发表时间：
1998-09-01
影响因子：
10.5
通讯作者：
Schüpbach, T
中科院分区：
文献类型：
--
作者：
Ghabrial, A;Ray, RP;Schüpbach, T
秋葵 (okr)、纺锤体-B (spnB) 和纺锤体-D (spnD) 是一组雌性不育基因座的三个成员，这些基因座会产生卵母细胞和卵子形态缺陷，包括蛋壳和胚胎中的可变背腹缺陷、卵泡细胞上皮和卵母细胞中的前后缺陷以及卵母细胞核异常 形态学。其中许多表型反映了 grk-Egfr 信号传导过程中的缺陷，并且可以通过未能积累野生型水平的 Gurken 和 Fs(1)K10 来解释。我们克隆了 okr 和 spnB，并表明 okr 编码酵母 DNA 修复蛋白 Rad54 的果蝇同源物，而 spnB 编码与减数分裂特异性 DMC1 基因相关的 Rad51 样蛋白。在对其在 DNA 修复中的作用进行功能测试时，我们发现 okr 的行为与其酵母同源物相似，因为它是有丝分裂和减数分裂细胞所必需的。相反，spnB 和 spnD 似乎仅在减数分裂中需要。参与减数分裂 DNA 代谢的基因对卵母细胞模式具有特定影响，这一事实意味着减数分裂细胞周期的进展与卵子发生过程中某些发育事件的调节相协调。
okra (okr), spindle-B (spnB), and spindle-D (spnD) are three members of a group of female sterile loci that produce defects in oocyte and egg morphology, including variable dorsal-ventral defects in the eggshell and embryo, anterior-posterior defects in the follicle cell epithelium and in the oocyte, and abnormalities in oocyte nuclear morphology. Many of these phenotypes reflect defects in grk-Egfr signaling processes, and can be accounted for by a failure to accumulate wild-type levels of Gurken and Fs(1)K10. We have cloned okr and spnB, and show that okr encodes the Drosophila homolog of the yeast DNA-repair protein Rad54, and spnB encodes a Rad51-like protein related to the meiosis-specific DMC1 gene. In functional tests of their role in DNA repair, we find that okr behaves like its yeast homolog in that it is required in both mitotic and meiotic cells. In contrast, spnB and spnD appear to be required only in meiosis. The fact that genes involved in meiotic DNA metabolism have specific effects on oocyte patterning implies that the progression of the meiotic cell cycle is coordinated with the regulation of certain developmental events during oogenesis.