Immunosuppressive activity of the ethanol extract of Siegesbeckia orientalis on the immune responses to ovalbumin in mice
Immunosuppressive activity of the ethanol extract of Siegesbeckia orientalis on the immune responses to ovalbumin in mice
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DOI：
10.1002/cbdv.200690077
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发表时间：
2006-01-01
影响因子：
2.9
通讯作者：
Wang, Hua
中科院分区：
文献类型：
--
作者：
Sun, Hong-Xiang;Wang, Hua
本文研究了Siegesbeckia orientalis乙醇提取物（EESO）对小鼠免疫功能的抑制作用。EESO显着抑制刀豆球蛋白A（Con A）和脂多糖（LPS）刺激的脾细胞增殖在体外以浓度依赖性的方式。在第0天和第14天用卵清蛋白（OVA）皮下免疫ICR小鼠。从免疫当天开始，以0.25、0.5和1.0 mg的单次剂量以7天的间隔腹膜内给予小鼠EESO。在第28天，测量血清中的OVA特异性抗体以及促分裂原和OVA诱导的脾细胞增殖。EESO能显著抑制Con A、LPS和OVA诱导的小鼠脾细胞增殖，并呈剂量依赖性。EESO还显著降低了OVA免疫小鼠中OVA特异性血清IgG、IgG 1和IgG 2b水平。此外，EESO在1.0 mg剂量下对IgG 1抗体的降低作用比环孢素A（CsA;阳性药物）更显著。结果表明，EESO能抑制卵清蛋白引起的小鼠细胞和体液免疫反应，作为免疫抑制剂值得进一步研究。
The in vitro and in vivo immunosuppressive activity of the ethanol extract of Siegesbeckia orientalis (EESO) was studied on the immune responses in mice. EESO significantly suppressed concanavalin A (Con A)- and lipopolysaccharide (LPS)-stimulated splenocyte proliferation in vitro in a concentration-dependent manner. ICR Mice were immunized subcutaneously with ovalbumin (OVA) on days 0 and 14. Beginning on the day of immunization, the mice were administered intraperitoneally with EESO at a single dose of 0.25, 0.5, and 1.0 mg at intervals of 7 days. On day 28, OVA-specific antibodies in serum, and mitogen- and OVA-induced splenocyte proliferation were measured. EESO significantly Suppressed Con A-, LPS- and OVA-induced splenocyte proliferation in the OVA-immunized mice in a dose-dependent manner. The OVA-specific serum IgG, IgG1, and IgG2b levels in the OVA-immunized mice were also significantly reduced by EESO. Moreover, reducing effect on the IgG1 antibody of EESO at the dose of 1.0 mg was more significant than that of cyclosporin A (CsA; positive drug). The results suggest that EESO could suppress the cellular and humoral response to ovalbumin in mice, and deserve further investigations to be developed as immunosuppressant.