Analysis of expressed sequence tags from Petunia flowers
Analysis of expressed sequence tags from Petunia flowers
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DOI：
10.1016/j.plantsci.2007.07.011
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发表时间：
2007-11-01
期刊：
影响因子：
5.2
通讯作者：
Ando, Toshio
中科院分区：
文献类型：
--
作者：
Shimamura, Katsuyoshi;Ishimizu, Takeshi;Ando, Toshio
从整个花部分（包括花蕾）、花粉和花粉管制备矮牵牛 cDNA 文库，以生成表达序列标签 (EST)。对7001个随机克隆进行单向测序，鉴定出611组相关序列和2410个单序列。对高度保守的 1098 个 EST 进行了功能分配。花粉和花粉管文库中经常发现编码参与钙依赖性信号通路和细胞壁代谢（如果胶降解和修饰）的蛋白质的 EST。来自芽/花cDNA文库的2976个cDNA克隆用于构建微阵列。 112 个功能注释的基因在花蕾即将开放之前上调，包括花青素色素沉着和蛋白质降解的基因。这些 EST 和微阵列将用于矮牵牛花性状的分析。 (C) 2007 Elsevier Ireland Ltd. 保留所有权利。
Petunia cDNA libraries were prepared from whole flower parts (including buds), pollen, and pollen tubes to generate expressed sequence tags (ESTs). A total of 7001 random clones were subjected to unidirectional sequencing, resulting in identification of 611 groups of related sequences and 2410 singletons. Highly conserved 1098 ESTs were functionally assigned. ESTs encoding proteins involved in the calcium-dependent signal pathway and in cell wall metabolism such as pectin degradation and modification were frequently found in the pollen and pollen tube libraries. The 2976 cDNA clones from the bud/flower cDNA libraries were used for the construction of microarrays. The 112 functionally annotated genes were up-regulated in the buds just before opening, including the genes for anthocyanin pigmentation and protein degradation. These ESTs and microarrays will serve the analysis of floral traits of petunias. (C) 2007 Elsevier Ireland Ltd. All rights reserved.