Antibody to carbonic anhydrase II is present in primary biliary cirrhosis (PBC) irrespective of antimitochondrial antibody status
Antibody to carbonic anhydrase II is present in primary biliary cirrhosis (PBC) irrespective of antimitochondrial antibody status
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无论抗线粒体抗体状态如何，原发性胆汁性肝硬化 (PBC) 中都存在碳酸酐酶 II 抗体
DOI：
10.1046/j.1365-2249.1998.00735.x
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发表时间：
1998
影响因子：
4.6
通讯作者：
M. Podda
中科院分区：
文献类型：
--
作者：
P. Invernizzi;P. Battezzati;A. Crosignani;P. Zermiani;M. Bignotto;N. Papa;M. Zuin;M. Podda
碳酸酐酶II（一种在胆管上皮中大量存在的酶）抗体已被提议作为抗线粒体抗体阴性PBC的诊断标志物。在这项研究中，我们确定了它的患病率和临床意义，在一个大系列的抗线粒体抗体阳性和阴性PBC患者。通过Western免疫印迹法检测了215例连续PBC患者（26例抗线粒体抗体阴性）、13例自身免疫性肝炎患者、25例原发性干燥综合征（pSS）患者、12例系统性硬化症患者、19例系统性红斑狼疮患者和73例健康受试者的血清对碳酸酐酶II的反应性。PBC中抗碳酸酐酶Ⅱ抗体（滴度1：100）的检出率为8%。在抗线粒体抗体阴性PBC中未发现对碳酸酐酶II的特异性反应性（7% vs 8%，抗线粒体抗体阳性PBC）。PBC中的腹水（P = 0.006）和干燥综合征（SS）（P =0.022）与抗体的存在显着相关。 在PBC相关SS患者中，患病率（19%）与pSS（16%）相似。当血清稀释度为1：40时，PBC阳性血清的患病率上升至27%，但疾病特异性降低。我们在大量PBC患者中的发现排除了抗碳酸酐酶II抗体的存在与抗线粒体抗体缺乏之间的关系。在阳性患者中发现腹水的发生率较高，需要进一步评估。
Antibody to carbonic anhydrase II, an enzyme abundantly present in biliary epithelium, has been proposed as a diagnostic marker for antimitochondrial antibody‐negative PBC. In this study we determine its prevalence and clinical significance in a large series of patients with antimitochondrial antibody‐positive and ‐negative PBC. Reactivity to carbonic anhydrase II was sought by Western immunoblotting in sera from 215 consecutive patients with PBC (26 antimitochondrial antibody‐negative), 13 with autoimmune hepatitis, 25 with primary Sjögren's syndrome (pSS), 12 with systemic sclerosis, 19 with systemic lupus erythematosus and 73 healthy subjects. The prevalence of antibody to carbonic anhydrase II (titre 1:100) in PBC was 8%. No specific reactivity to carbonic anhydrase II was found in antimitochondrial antibody‐negative PBC (7% versus 8% in antimitochondrial antibody‐positive PBC). Ascites (P = 0.006) and Sjögren's syndrome (SS) (P = 0.022) in PBC were significantly associated with presence of the antibody. In patients with SS associated with PBC, the prevalence (19%) was similar to that observed in pSS (16%). At a serum dilution of 1:40, the prevalence of positive sera in PBC rose to 27% but disease specificity was reduced. Our findings in a large population of PBC patients rule out a relation between presence of antibody to carbonic anhydrase II and lack of antimitochondrial antibody. The higher prevalence of ascites found in positive patients warrants further evaluation.
影响因子：
4.4
作者：
I. Nishimori;T. Bratanova;I. Toshkov;T. Caffrey;M. Mogaki;Y. Shibata;Michael A. Hollingsworth
通讯作者：
I. Nishimori;T. Bratanova;I. Toshkov;T. Caffrey;M. Mogaki;Y. Shibata;Michael A. Hollingsworth
免疫介导的胆管炎啮齿动物模型的开发和表征。
DOI：
10.1073/pnas.93.1.216
发表时间：
1996
影响因子：
11.1
作者：
Ueno,Y;Phillips,JO;Ludwig,J;Lichtman,SN;LaRusso,NF
通讯作者：
LaRusso,NF