Translocation of Tektin3 to the equatorial segment of heads in bull spermatozoa exposed to dibutyryl cAMP and calyculin A
Translocation of Tektin3 to the equatorial segment of heads in bull spermatozoa exposed to dibutyryl cAMP and calyculin A
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Tektin3 易位至暴露于二丁酰 cAMP 和花萼蛋白 A 的公牛精子头部赤道段
DOI：
10.1002/mrd.22763
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发表时间：
2017
期刊：
影响因子：
2.5
通讯作者：
Iida H
中科院分区：
文献类型：
--
作者：
Tsukamoto M;Hiyama E;Hirotani K;Gotoh T;Inai T;Iida H
Tektins(TEKTs)是一种丝状蛋白，与纤毛、鞭毛、基底体和中心粒中的微管有关。5种TEKT(TEKT1、-2、-3、-4和-5)已被鉴定为哺乳动物精子鞭毛的成分。我们先前报道，TKET1和-3也存在于啮齿动物精子的头部。本研究清楚地表明，在未激活的公牛精子中，TEKT2存在于顶体帽，而TEKT3仅存在于精子头顶体后区域的质膜之下，这是建立在TEKT1、-2和-3在精子头上的分布差异的基础上的。我们还发现，细胞通透性cAMP和蛋白磷酸酶抑制剂Calyculin A对公牛精子的过度激活促进了TEKT3从顶体后区域到精子头的赤道段的移位，而在顶体反应中积累在赤道段的TEKT3丢失了。因此，在公牛精子中，TEKT3移位到赤道段可能是一种与获能或过度激活相关的现象。是Reprod。戴夫。2017年84：30-43。©2016 Wiley期刊，Inc.
Tektins (TEKTs) are filamentous proteins associated with microtubules in cilia, flagella, basal bodies, and centrioles. Five TEKTs (TEKT1, ‐2, ‐3, ‐4, and ‐5) have been identified as components of mammalian sperm flagella. We previously reported that TKET1 and ‐3 are also present in the heads of rodent spermatozoa. The present study clearly demonstrates that TEKT2 is present at the acrosome cap whereas TEKT3 resides just beneath the plasma membrane of the post‐acrosomal region of sperm heads in unactivated bull spermatozoa, and builds on the distributional differences of TEKT1, ‐2, and ‐3 on sperm heads. We also discovered that hyperactivation of bull spermatozoa by cell‐permeable cAMP and calyculin A, a protein phosphatase inhibitor, promoted translocation of TEKT3 from the post‐acrosomal region to the equatorial segment in sperm heads, and that TEKT3 accumulated at the equatorial segment is lost upon acrosome reaction. Thus, translocation of TEKT3 to the equatorial segment may be a capacitation‐ or hyperactivation‐associated phenomenon in bull spermatozoa.Mol. Reprod. Dev. 84: 30–43, 2017. © 2016 Wiley Periodicals, Inc.