Imaging of plant dynamin-related proteins and clathrin around the plasma membrane by variable incidence angle fluorescence microscopy
Imaging of plant dynamin-related proteins and clathrin around the plasma membrane by variable incidence angle fluorescence microscopy
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DOI：
10.5511/plantbiotechnology.24.449
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发表时间：
2007-12-01
影响因子：
1.6
通讯作者：
Tsutsumi, Nobuhiro
中科院分区：
文献类型：
--
作者：
Fujimoto, Masaru;Arimura, Shin-ichi;Tsutsumi, Nobuhiro
内吞作用是真核细胞的一种基本现象。在动物细胞中，发动蛋白和网格蛋白在胞吞过程中的囊泡形成中起核心作用，但植物中类似蛋白的作用还不太清楚。利用可变入射角荧光显微镜（VIAFM）观察了GFP标记的拟南芥动力蛋白相关蛋白（DRP 1A和DRP 2B）和网格蛋白轻链（AtCLC）在烟草悬浮细胞质膜周围的定位模式和行为。DRP 1A、DRP 2B和AtCLC的GFP融合物被观察为直径为200-500 nm的点状斑点。斑点移向和远离细胞表面或组装和拆卸。斑点的定位模式和行为与先前报道的动物动力蛋白和网格蛋白信号相似。这些结果提高了DRP 1A，DRP 2B和AtCLC参与质膜周围的膜运输，包括内吞作用的可能性。
Endocytosis is an essential phenomenon in eukaryotic cells. In animal cells, dynamin and clathrin play central roles in vesicle formation in the process of endocytosis, but the roles of similar proteins in plants are less well understood. Here, we observed the localization pattern and behavior of GFP-labeled Arabidopsis dynamin-related proteins (DRP1A and DRP2B), and clathrin light chain (AtCLC) around the plasma membrane in tobacco suspension cells by using variable incidence angle fluorescence microscopy (VIAFM). GFP fusions of DRP1A, DRP2B and AtCLC were observed as dot-like puncta 200-500 nm in diameter. The puncta moved to and away from the cell surface or also assembled and disassembled. The localization pattern and behavior of the puncta were similar to those of animal dynamin and clathrin signals reported previously. These results raise the possibility that DRP1A, DRP2B and AtCLC are involved in membrane trafficking around the plasma membrane, including endocytosis.