Neighbourhood Continuity Is Not Required for Correct Testis Gene Expression in Drosophila
Neighbourhood Continuity Is Not Required for Correct Testis Gene Expression in Drosophila
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DOI：
10.1371/journal.pbio.1000552
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发表时间：
2010-11-01
期刊：
影响因子：
9.8
通讯作者：
Russell, Steven
中科院分区：
文献类型：
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作者：
Meadows, Lisa A.;Chan, Yuk Sang;Russell, Steven
现在人们普遍认为真核生物基因组中的基因组织是非随机的，并且提出这种组织对于基因表达和基因组进化可能是重要的。特别是，从酵母到人类的一系列生物体中的几个大规模基因表达分析的结果表明，具有相似组织特异性或时间表达谱的基因组在基因表达邻域中聚集在基因组内。虽然邻里关系的存在是明确的，这方面的基因组组织的根本原因目前还不清楚，有很少的实验证据，解决邻里组织的基因组结构。我们报告的三个明确定义的男性特定的基因表达的果蝇基因组中的街区的精确映射的染色体倒位的合成有针对性的中断。我们使用全转录组微阵列分析比较了携带反转染色体的个体与其非反转但在其他方面相同的祖细胞的基因表达，并使用特定的定量实时PCR分析验证了这些数据。对于每个邻域，我们生成并检查多个反演。我们没有发现定义每个社区的基因表达的显着差异。我们进一步表明，反演空间分离的两个一半的邻居在核中。因此，模型解释邻里组织的局部序列相互作用，增强子串扰，或短程染色质的影响是不太可能占这方面的基因组组织。我们的研究挑战的概念，至少在睾丸的情况下，表达邻域是正确的基因表达所必需的真核基因组组织的一个功能。
It is now widely accepted that gene organisation in eukaryotic genomes is non-random and it is proposed that such organisation may be important for gene expression and genome evolution. In particular, the results of several large-scale gene expression analyses in a range of organisms from yeast to human indicate that sets of genes with similar tissue-specific or temporal expression profiles are clustered within the genome in gene expression neighbourhoods. While the existence of neighbourhoods is clearly established, the underlying reason for this facet of genome organisation is currently unclear and there is little experimental evidence that addresses the genomic requisites for neighbourhood organisation. We report the targeted disruption of three well-defined male-specific gene expression neighbourhoods in the Drosophila genome by the synthesis of precisely mapped chromosomal inversions. We compare gene expression in individuals carrying inverted chromosomes with their non-inverted but otherwise identical progenitors using whole-transcriptome microarray analysis, validating these data with specific quantitative real-time PCR assays. For each neighbourhood we generate and examine multiple inversions. We find no significant differences in the expression of genes that define each of the neighbourhoods. We further show that the inversions spatially separate both halves of a neighbourhood in the nucleus. Thus, models explaining neighbourhood organisation in terms of local sequence interactions, enhancer crosstalk, or short-range chromatin effects are unlikely to account for this facet of genome organisation. Our study challenges the notion that, at least in the case of the testis, expression neighbourhoods are a feature of eukaryotic genome organisation necessary for correct gene expression.