An improved gas chromatographic method for measuring glucosamine and muramic acid concentrations.
An improved gas chromatographic method for measuring glucosamine and muramic acid concentrations.
复制标题
一种改进的气相色谱法，用于测量葡萄糖胺和胞壁酸浓度。
DOI：
10.1016/0003-2697(83)90398-6
复制
发表时间：
1983
影响因子：
2.9
通讯作者：
Newell,SY
中科院分区：
文献类型：
--
作者：
Hicks,RE;Newell,SY
建立了一种同时测定沼泽草凋落物中氨基葡萄糖和胞壁酸含量的方法。互花米草样品用丙酮预提取以除去含有氨基糖的脂质，然后在6 n HCl（100°C，4.5 h）中水解。水解液中氨基糖经离子交换层析分离，回收率>90%，重复性好（CV <5%）。将分离的氨基糖转化为O-甲基肟乙酸酯。加入β-苯基葡萄糖和N-甲基葡糖胺作为内标。通过毛细管柱气相色谱法定量样品衍生物。OV-101和SE-54毛细管柱能将氨基葡萄糖和胞壁酸与其它氨基糖完全分离。氨基葡萄糖和胞壁酸在气相色谱分析中的检测限低于30 pmol，使用分流模式注射（SE-54柱）。丝状真菌和原核生物量可以估计同时通过使用葡糖胺和胞壁酸生物量转换因子结合这种方法。
A method of simultaneously measuring glucosamine and muramic acid concentrations in marsh grass litter was developed. Spartina alterniflora samples were preextracted with acetone to remove lipids containing amino sugars and then hydrolyzed in 6 n HCl (100°C, 4.5 h). Amino sugars in the hydrolysates were isolated by ion-exchange chromatography, which gave good recoveries (>90%) and reproducibility (CV <5%). Isolated amino sugars were converted to O-methyloxime acetates. β-Phenylglucose and N-methylglucamine were added as internal standards. Sample derivatives were quantified by capillary column gas chromatography. OV-101 and SE-54 capillary columns completely separated glucosamine and muramic acid from other amino sugars. The detection limit of glucosamine and muramic acid during gas chromatographic analysis was below 30 pmol using splitless-mode injection (SE-54 column). Filamentous fungal and procaryotic biomasses may be estimated simultaneously by using glucosamine and muramic acid biomass conversion factors in conjunction with this method.