Thermodynamic model of bacterial transcription.
Thermodynamic model of bacterial transcription.
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DOI：
10.1103/physreve.106.044406
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发表时间：
2022-10
影响因子：
2.4
通讯作者：
Finzi, Laura
中科院分区：
文献类型：
--
作者：
Qian, Jin;Dunlap, David;Finzi, Laura
转录暂停高度受模板DNA和新生转录物序列的调节。在这里，我们提出了一个热力学模型的转录暂停，转录气泡和新生RNA结构的热能的基础上，描述的动力学之间的反应途径主动易位，元素，回溯，和发夹稳定的暂停。该模型很容易预测实验检测暂停高分辨率光镊测量转录。与其他模型不同，它还预测了张力和GreA转录因子对暂停的影响。
Transcriptional pausing is highly regulated by the template DNA and nascent transcript sequences. Here, we propose a thermodynamic model of transcriptional pausing, based on the thermal energy of transcription bubbles and nascent RNA structures, to describe the kinetics of the reaction pathways between active translocation, elemental, backtracked, and hairpin-stabilized pauses. The model readily predicts experimentally detected pauses in high-resolution optical tweezers measurements of transcription. Unlike other models, it also predicts the effect of tension and the GreA transcription factor on pausing.