Universal hybridization using LNA (locked nucleic acid) containing a novel pyrene LNA nucleotide monomer
Universal hybridization using LNA (locked nucleic acid) containing a novel pyrene LNA nucleotide monomer
复制标题
DOI：
10.1039/b106065c
复制
发表时间：
2001-01-01
影响因子：
4.9
通讯作者：
Wengel, J
中科院分区：
文献类型：
--
作者：
Babu, BR;Wengel, J
新型芘 LNA 核苷酸单体在掺入 DNA 链或 2'-OMe-RNA/LNA 嵌合链时可介导通用杂交。对于后者，记录了高亲和力通用杂交，而不损害通用芘 LNA 核苷酸单体附近碱基的碱基配对选择性。
A novel pyrene LNA nucleotide monomer is shown to mediate universal hybridization when incorporated into a DNA strand or a 2'-OMe-RNA/LNA chimeric strand. For the latter, high-affinity universal hybridization without compromising the base-pairing selectivity of bases neighbouring the universal pyrene LNA nucleotide monomer is documented.