Acidocella aluminiidurans sp nov., an aluminium-tolerant bacterium isolated from Panicum repens grown in a highly acidic swamp in actual acid sulfate soil area of Vietnam
Acidocella aluminiidurans sp nov., an aluminium-tolerant bacterium isolated from Panicum repens grown in a highly acidic swamp in actual acid sulfate soil area of Vietnam
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DOI：
10.1099/ijs.0.011569-0
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发表时间：
2010-04-01
影响因子：
2.8
通讯作者：
Sunairi, Michio
中科院分区：
文献类型：
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作者：
Kimoto, Ken-ichiro;Aizawa, Tomoko;Sunairi, Michio
从生长在酸性沼泽（pH 3）中的一种水草（Panicumrepens）中分离到一株耐铝细菌，菌株AL 46（T）。细胞为革兰氏阴性、需氧、不形成孢子、不活动的杆状（0.3 μ m宽，1.2-1.6 μ m长）。16 S rRNA基因序列分析表明，菌株AL 46（T）属于α-变形菌纲（Alphaproteobacteria）酸胞菌属（Acidocella）。菌株AL 46（T）与Acidocellafacilis和Acidocellaminolytica的模式菌株最接近（16 S rRNA基因序列相似性分别为99.4%和97.8%）。菌株AL 46（T）与上述模式菌株的DNA-DNA同源性为40%。生理生化试验结果表明，新菌株与两种已知的酸胞菌属菌株在表型上存在差异。主要的细胞脂肪酸（环丙基C-19：0和C-18：1），主要的类异戊二烯醌（Q-10）和DNA G+C含量（65.6摩尔%）的数据是根据那些报道的属Acidocella。因此，菌株AL 46（T）被认为代表了酸胞菌属的一个新种，并建议将其命名为耐铝酸胞菌（Acidocella aluminidurans sp. nov.）。模式菌株为AL 46（T）（=NBRC 104303（T）=VTCC-D9-1（T））。
An aluminium-tolerant bacterium, strain AL46(T), was isolated from a waterweed, Panicum repens, grown in a highly acidic swamp (pH 3) at an actual acid sulfate soil area of Vietnam. Cells were Gram-negative, aerobic, non-spore-forming, non-motile rods (0.3 mu m wide and 1.2-1.6 mu m long). 16S rRNA gene sequence analysis indicated that strain AL46(T) belongs to the genus Acidocella, class Alphaproteobacteria. Strain AL46(T) was related most closely to the type strains of Acidocella facilis and Acidocella aminolytica (99.4 and 97.8% 16S rRNA gene sequence similarity, respectively). Levels of DNA-DNA relatedness between strain AL46(T) and the above type strains were 40%. The results of physiological and biochemical tests allowed the novel strain to be differentiated phenotypically from the two recognized Acidocella species. Data for predominant cellular fatty acids (cyclopropyl C-19:0 and C-18:1), major isoprenoid quinone (Q-10) and DNA G+C content (65.6 mol%) were in accordance with those reported for the genus Acidocella. Therefore, strain AL46(T) is considered to represent a novel species of the genus Acidocella, for which the name Acidocella aluminiidurans sp. nov. is proposed. The type strain is AL46(T) (=NBRC 104303(T) =VTCC-D9-1(T)).