Hepatoprotective Angelica sinensis silver nanoformulation against multidrug resistant bacteria and the integration of multicomponent logic gate system
Hepatoprotective Angelica sinensis silver nanoformulation against multidrug resistant bacteria and the integration of multicomponent logic gate system
复制标题
当归银纳米制剂对抗多重耐药菌的保肝作用及多组分逻辑门系统的集成
作者：
Jouharsha Afthab;Nafeesa Khatoon;Lulu Zhou;Tianming Yao;Shuo Shi
抗生素的滥用导致了毒性的出现，特别是肝毒性和微生物耐药性的出现。因此，一系列新的保肝，生物相容性，抗菌银纳米制剂（AS-AgNPs）开发利用重要的中药植物当归。采用紫外-可见光谱、透射电子显微镜（TEM）、扫描电子显微镜（SEM）、X射线衍射（XRD）和傅里叶变换红外光谱（FT-IR）对不同粒径的AS-AgNPs进行了表征。研究了AS-AgNPs对革兰氏阳性菌、革兰氏阴性菌和多重耐药菌的尺寸依赖性抗菌性能。发现具有不同尺寸的AS-AgNP对六种细菌的最小抑制浓度（MIC）在5-100 μg mL-1的范围内，直到12个循环都没有抗性。处理后细菌的TEM和SEM结果表明，AS-AgNPs通过产生孔隙来破坏细胞膜。针对HepG 2细胞系评估了AS-AgNP的细胞相容性和细胞保护作用，结果显示，高达100 μg mL−1的浓度下，85%的细胞存活，AST和ALT水平几乎没有变化。此外，通过基于AS-AgNP 1与细菌之间的相互作用整合多个组件，设计了包括基本逻辑门（AND，OR，NOR，INHIBIT，IMPLICATION和YES）、三个输入逻辑门（OR，和NOR）和组合门（INH-OR，INH-YES，INH-INH，AND-NOR和NOT-AND-NOR）的逻辑组合库，其中DiSC 3（5）用作信号报告子。该系统清楚地展示了简单逻辑电路执行复杂分析以检测多种细菌的能力。
The rampant usage of antibiotics has led to the emergence of toxicity, especially hepatotoxicity and the emergence of microbial drug resistance. Hence, a series of novel hepatoprotective, biocompatible, antibacterial silver nanoformulations (AS-AgNPs) were developed by using the important Chinese medicinal plant Angelica sinensis. The different size of AS-AgNPs were characterized by UV-Visible spectroscopy, transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR). The size-dependent antibacterial properties of AS-AgNPs were investigated against Gram-positive, Gram-negative and multi-drug resistant bacteria. The minimum inhibitory concentration (MIC) of AS-AgNPs with different size against six bacteria was found to be in the range of 5–100 μg mL−1 with no resistance till 12 cycles. TEM and SEM results of bacteria after the treatment suggested that AS-AgNPs disrupted the cell membrane by creating pores. The cytocompatibility and cytoprotective effect of AS-AgNPs were evaluated against HepG2 cell lines, which showed that 85% of cells were viable up to 100 μg mL−1 of the concentration with almost no change in AST and ALT levels. Further, a logic combinatorial library, including basic logic gates (AND, OR, NOR, INHIBIT, IMPLICATION, and YES), three input logic gates (OR, and NOR) and combinatorial gates (INH-OR, INH-YES, INH-INH, AND-NOR, and NOT-AND-NOR) were designed by integrating multi-components based on the interaction between AS-AgNP1 and bacteria, where DiSC3(5) was used as the signal reporter. This system clearly demonstrates the ability of simple logic circuits to perform sophisticated analysis for the detection of multiple bacteria.