Rapid urine antibody test for Helicobacter pylori infection in adolescents
Rapid urine antibody test for Helicobacter pylori infection in adolescents
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青少年幽门螺杆菌感染的快速尿液抗体检测
DOI：
10.1111/ped.13286
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发表时间：
2017
影响因子：
1.4
通讯作者：
Kikuchi Shogo
中科院分区：
文献类型：
--
作者：
Okuda Masumi;Mabe Katsuhiro;Lin Yingsong;Chaochen Wang;Taniguchi Yohei;Kato Mototsugu;Kikuchi Shogo
背景幽门螺杆菌感染与胃癌有关；因此，早期诊断和治疗至关重要。thatH。幽门感染在青少年或青壮年很少有症状，筛选试验是必要的这一人群。在本研究中，快速尿h。评估幽门螺杆菌抗体（u - HpAb），并与尿和血清h进行比较。初中生幽门酶联免疫吸附试验（分别为u - HpELISA和s - HpELISA）。方法对Sasayama市1225名初中在校生进行问卷调查。检测尿液和血液样本的抗- H水平。幽门免疫球蛋白G抗体和快速u - HpAb由三位研究者独立进行。当所有研究者意见一致时，测试结果被确定为阳性或阴性。非一致性定义为未确定。结果共有187名学生参加了本次研究，并提供了尿样和血样。3名学生u - HpAb快速检测结果不确定。除上述结果外，u‐HpAb的阳性率为3.3%(6/184)，而u‐HpELISA和s‐HpELISA的阳性率分别为4.8%（10/187）和5.9%（11/187）。以s‐HpELISA和u‐HpELISA为标准，排除未确定的快速u‐HpAb结果，快速u‐HpAb的敏感性、特异性、阳性预测值和阴性预测值分别为85.7%、100%、100%和99.4%。结论尿- HpAb快速检测具有良好的特异性，但敏感性较低。
BackgroundHelicobacter pyloriinfection is associated with gastric cancer; thus, early diagnosis and treatment are crucial. Given thatH. pyloriinfection in adolescents or young adults has few symptoms, screening tests are necessary for this population. In this study, the accuracy of the rapid urineH. pyloriantibody (u‐HpAb) test was evaluated and compared with that of urine and serumH. pylorienzyme‐linked immunosorbent assay (u‐HpELISA and s‐HpELISA, respectively) in junior high school students.MethodsAll 1,225 students attending the junior high schools in Sasayama City were invited to participate in this study. Urine and blood samples were assayed for anti‐H. pyloriimmunoglobulin G antibodies, and rapid u‐HpAb was performed by three investigators independently. When all investigators were in agreement, the test was confirmed as positive or negative. Non‐concordance was defined as undetermined.ResultsIn total, 187 students participated in this study and provided both urine and blood samples. Three students had undetermined rapid u‐HpAb. Excluding these results, the positivity rate of rapid u‐HpAb was 3.3% (6/184), whereas that for u‐HpELISA and s‐HpELISA was 4.8% (10/187) and 5.9% (11/187), respectively. Using s‐HpELISA and u‐HpELISA as the standards, the sensitivity, specificity, positive predictive value, and negative predictive value of rapid u‐HpAb were 85.7%, 100%, 100%, and 99.4%, respectively, excluding the undetermined rapid u‐HpAb results.ConclusionsRapid urine‐HpAb test had excellent specificity but relatively low sensitivity.