Urine drug testing for opioids, cocaine, and metabolites by direct injection liquid chromatography/tandem mass spectrometry
Urine drug testing for opioids, cocaine, and metabolites by direct injection liquid chromatography/tandem mass spectrometry
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DOI：
10.1002/rcm.1098
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发表时间：
2003-01-01
影响因子：
2
通讯作者：
Huestis, MA
中科院分区：
文献类型：
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作者：
Dams, R;Murphy, CM;Huestis, MA
建立了同时测定尿中阿片类药物、可卡因和代谢物的LC/MS/MS方法，并对其进行了验证。将10等分的尿液直接注入LC/MS/MS系统。样品制备的缺乏大大减少了总分析时间。采用反相色谱梯度洗脱，分离时间为26min。常压化学电离是一种坚固高效的碱性药物电离技术。在选择性反应监测(SRM)的基础上进行鉴定和定量。通过线性回归分析(加权系数1/x)，用氚代内标物进行校准。方法的最低定量限(LOQ)为10-100 ng/mL，线性范围最大为10000 ng/mL，平均相关系数(R-2)为0.99。满足了特异性、精密度、准确度、稀释完整性、基质效应和稳定性的分析验证标准。该方法简单、省时，适用于国家药物滥用研究所(NIDA)临床研究项目中的非法药物使用监测和美沙酮治疗依从性。2003年由John Wiley Sons，Ltd.出版。
A sensitive and specific liquid chromatography/tandem mass spectrometry (LC/MS/MS) method for the simultaneous quantification of opioids, cocaine, and metabolites in urine was developed and validated. A 10-muL aliquot of urine was injected directly onto the LC/MS/MS system. The lack of sample preparation substantially reduced total analysis time. Separation was performed by reversed-phase chromatography with gradient elution for all analytes in 26 min. Atmospheric pressure chemical ionization (APCI) was a rugged and efficient ionization technique for basic drugs. Identification and quantification was based on selected reaction monitoring (SRM). Calibration, with deuterated internal standards, was performed by linear regression analysis (weighting factor 1/x). Limits of quantitation (LOQ) were established between 10-100ng/mL and linearity was obtained up to a maximum of 10 000 ng/mL with an average correlation coefficient (R-2) > 0.99. Analytical validation criteria for specificity, precision, accuracy, dilution integrity, matrix effect, and stability were fulfilled. The method proved to be simple and time efficient, and was applicable for illicit drug use monitoring and methadone treatment compliance in clinical research projects at the National Institute on Drug Abuse (NIDA). Published in 2003 by John Wiley Sons, Ltd.