Generation of hybridomas secreting murine reaginic antibodies of anti-DNP specificity.
Generation of hybridomas secreting murine reaginic antibodies of anti-DNP specificity.
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产生分泌抗DNP特异性鼠反应素抗体的杂交瘤。
DOI：
10.4049/jimmunol.124.2.775
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发表时间：
1980
影响因子：
4.4
通讯作者：
T. Waks
中科院分区：
文献类型：
--
作者：
Z. Eshhar;M. Ofarim;T. Waks
通过免疫细胞过继转移富集的脾淋巴细胞与 NSI 浆细胞瘤细胞系的融合，产生高频率分泌大量反应性抗 DNP 抗体的杂交瘤系。鼠IgE分子以高亲和力结合DNP，并通过免疫吸附特异性纯化。 190,000 道尔顿分子由表观分子量的 lambda 轻链和 epsilon 重链组成。约90,000。抗类特异性抗血清的吸收研究表明，一些杂合克隆除了ε重链之外还分泌由lambda-和κ-轻链组成的混合分子。单克隆反应素抗体与肥大细胞或大鼠嗜碱性白血病细胞结合，并在与 DNP 蛋白结合后引发过敏反应。
High frequency of hybridoma lines secreting large amounts of reaginic anti-DNP antibodies were generated by the fusion of splenic lymphocytes enriched by adoptive transfer of immune cells and the NSI plasmacytoma cell line. The murine IgE molecule binds DNP with high affinity and was specifically purified by immunoabsorption. The 190,000 dalton molecule is composed of a lambda-light chain and an epsilon-heavy chain of an apparent m.w. of about 90,000. Absorption studies with anti-class-specific antisera suggest that some of the hybrid clones secrete also a mixed molecule consisting of lambda- and kappa-light chains in addition to the epsilon-heavy chain. The monoclonal reaginic antibody binds to mast cells or rat basophilic leukemia cells and, upon binding of DNP-protein, triggers an anaphylactic reaction.