Cloning, expression, and characterization of a gene encoding the human angiotensin II type 1A receptor.
Cloning, expression, and characterization of a gene encoding the human angiotensin II type 1A receptor.
复制标题
编码人血管紧张素 II 1A 型受体的基因的克隆、表达和表征。
DOI：
10.1016/s0006-291x(05)80804-6
复制
发表时间：
1992
影响因子：
3.1
通讯作者：
Fu
中科院分区：
文献类型：
--
作者：
C. Mauzy;O. Hwang;A. Egloff;Lan‐Hsin Wu;Fu
从人白细胞基因组文库中克隆了人血管紧张素II(ALL)1a型受体基因及其上游调控序列。启动子元件CAAT和TATA序列分别位于翻译起始点上游的−602和−538。推导的蛋白序列与大鼠和牛AT1a受体的同源性分别为94.7%和95.3%。表达的基因具有高亲和力的ALL和Dup753结合，并在功能上与肌醇磷酸转换偶联。Northern分析显示AT1受体在胎盘、肺、心脏、肝脏和肾脏中均有表达。用5‘端未翻译和编码的序列作为探针进行Southern印迹分析，证实人类基因组中存在另一种AT1亚型。
The human angiotensin II (All) type 1a receptor gene and its upstream control sequence has been cloned from a human leukocyte genomic library. The promoter element CAAT and TATA sequences were found at −602 and −538, respectively, upstream from the translational initiation site. The deduced protein sequence is homologous to rat and bovine AT1areceptors (94.7% and 95.3% identity). The expressed gene exhibited high-affinity All and Dup753 binding and was functionally coupled to inositol phosphate turnover. Northern analysis of human tissues showed AT1receptor mRNA expression in placenta, lung, heart, liver, and kidney. Using 5′ untranslated and coding sequence as probes in a Southern blot analysis, it was established that another AT1subtype exists in the human genome.