Imaging voltage and brain chemistry with genetically encoded sensors and modulators.
Imaging voltage and brain chemistry with genetically encoded sensors and modulators.
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DOI：
10.1016/j.cbpa.2020.07.006
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发表时间：
2020-08
影响因子：
7.8
通讯作者：
Akash Pal;Lin Tian
中科院分区：
文献类型：
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作者：
Akash Pal;Lin Tian
神经元和神经胶质在功能上被组织成回路和高阶结构，允许复杂行为所需的精确信息处理。为了更好地了解大脑的结构和功能，我们必须了解突触的连接，动作电位的产生和传播，以及精心安排的分子信号。最近，电压、细胞内钙和神经递质/调节剂传感器的显著改进，结合先进的显微镜技术，为清醒行为动物的细胞和电路活动的活体解剖提供了新的机会。本文综述了分子和细胞事件基因编码传感器的最新发展趋势及其在健康和疾病神经系统研究中的潜在应用。
Neurons and glia are functionally organized into circuits and higher-order structures that allow the precise information processing required for complex behaviors. To better understand the structure and function of the brain, we must understand synaptic connectivity, action potential generation and propagation, as well as well-orchestrated molecular signaling. Recently, dramatically improved sensors for voltage, intracellular calcium, and neurotransmitters/modulators, combined with advanced microscopy provide new opportunities forin vivodissection of cellular and circuit activity in awake, behaving animals. This review focuses on the current trends in genetically encoded sensors for molecules and cellular events and their potential applicability to the study of nervous system in health and disease.