Role of the tomato fruit ripening regulator MADS-RIN in resistance to Botrytis cinerea infection
Role of the tomato fruit ripening regulator MADS-RIN in resistance to Botrytis cinerea infection
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DOI：
10.1093/fqsafe/fyab028
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发表时间：
2021-10
影响因子：
5.6
通讯作者：
Hui Zheng;R. Jin;Zimeng Liu;Cui Sun;Yanna Shi;D. Grierson;Changqing Zhu;Shan Li;I. Ferguson-I.
中科院分区：
文献类型：
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作者：
Hui Zheng;R. Jin;Zimeng Liu;Cui Sun;Yanna Shi;D. Grierson;Changqing Zhu;Shan Li;I. Ferguson-I.
番茄MADS-RIN（RIN）转录因子是调节果实成熟的主要激活因子。近年来的研究表明，除了激活许多细胞壁基因外，它还抑制与果肉过度软化和细胞壁降解正相关的XTH 5、XTH 8和MAN 4a的表达，这可能表明它在成熟果实的抗病性中具有潜在的作用。本研究以野生型（WT）和RIN基因敲除（RIN-KO）突变体番茄果实为材料，接种灰葡萄孢菌（Botrytis cinerea），研究RIN基因在番茄成熟过程中对病原菌侵染的防御作用。结果表明，RIN-KO果实对B.cinerea侵染的敏感性更高，病斑面积更大。转录组数据和qRT-PCR分析表明，RIN-KO果实中苯丙氨酸解氨酶（PAL）和几丁质酶（CHI）基因表达量减少，相应酶活性降低。RIN-KO果实中编码病程相关蛋白（PRs）的基因PR 1a、PRSTH 2和编码APETALA 2/乙烯反应因子（AP 2/ERF）的基因ERF.A1、Pti 5、Pti 6、ERF.A4的转录水平均低于WT果实。此外，在不存在RIN的情况下，已报道编码细胞壁修饰酶XTH 5、XTH 8、MAN 4a的基因的表达升高，这可能与细胞壁性质相关。当存在时，RIN通过激活ERF.F4（II类（阻遏物类）ERF基因家族成员）和ERF.F5来阻遏XTH 5的转录。这些结果支持了RIN通过上调抗病基因和防御酶活性以及减少编码某些细胞壁酶的转录本的积累来增强成熟相关的抗灰霉病感染的结论。
Tomato MADS-RIN (RIN) transcription factor has been shown to be a master activator regulating fruit ripening. Recent studies have revealed that in addition to activating many other cell wall genes, it also represses expression of XTH5, XTH8 and MAN4a, which are positively related to excess flesh softening and cell wall degradation, which might indicate it has a potential role in pathogen resistance of ripening fruit. In this study, both wild type (WT) and RIN-knockout (RIN-KO) mutant tomato fruit were infected with Botrytis cinerea, to investigate the function of RIN in defence against pathogen infection during ripening. The results showed that RIN-KO fruit were much more sensitive to B.cinerea infection with larger lesion sizes. Transcriptiome data and qRT-PCR assay indicate genes of phenylalanine ammonialyase (PAL) and chitinase (CHI) in RIN-KO fruit were reduced and their corresponding enzyme activities were decreased. Transcripts of genes encoding pathogenesis-related proteins (PRs), including PR1a, PRSTH2 and APETALA2/Ethylene Response Factor (AP2/ERF) including ERF.A1, Pti5, Pti6, ERF.A4 were reduced in RIN-KO fruit comparing to WT fruit. Moreover, in the absence of RIN the expression of genes encoding cell wall modifying enzymes XTH5, XTH8, MAN4a has been reported to be elevated, which is potentially correlated with cell wall properties. When present, RIN represses transcription of XTH5 by activating ERF.F4 a class II (repressor class) ERF gene family member and ERF.F5. These results support the conclusion that RIN enhances ripening-related resistance to grey mould infection by upregulating pathogen-resistance genes and defense enzyme activies as well as reducing accumulation of transcripts encoding some cell wall enzymes.