CONSTRUCTION OF A GENETIC LINKAGE MAP FOR PORPHYRA HAITANENSIS (BANGIALES, RHODOPHYTA) BASED ON SEQUENCE‐RELATED AMPLIFIED POLYMORPHISM AND SIMPLE SEQUENCE REPEAT MARKERS 1
CONSTRUCTION OF A GENETIC LINKAGE MAP FOR PORPHYRA HAITANENSIS (BANGIALES, RHODOPHYTA) BASED ON SEQUENCE‐RELATED AMPLIFIED POLYMORPHISM AND SIMPLE SEQUENCE REPEAT MARKERS 1
复制标题
DOI：
10.1111/j.1529-8817.2010.00855.x
复制
发表时间：
2010-08
影响因子：
2.9
通讯作者：
Chaotian Xie;Chang-sheng Chen;Xu Yan;J. Dehua
中科院分区：
文献类型：
--
作者：
Chaotian Xie;Chang-sheng Chen;Xu Yan;J. Dehua
分子标记和分子遗传图谱是任何植物进行分子育种的先决条件。人工栽培的坛紫菜T.J.Chang et B.F.Zeng的遗传连锁图谱尚未建立。本研究以青杨YSIII(野生型)与RTPM(红色型人工色素突变体)杂交产生的157个双单倍体(DH)系为作图群体。利用60对序列相关扩增多态性(SRAP)和39对简单序列重复(SSR)引物对双亲进行了多态性检测。选用15对SRAP和16对SSR多态引物对该群体进行了遗传分析。构建了总长830.6 cM、标记间平均10.13 cM的5个连锁群的67个SRAP标记和20个SSR标记的连锁遗传图谱。标记在各连锁群中分布均匀，无聚类现象。连锁群由12-23个标记组成，长度在134.2~197.3 cM之间。海棠基因组全长为942.4 cM，覆盖率为88.1%。这是首次报道了海滩松的完整遗传图谱。该图谱为海棠高密度遗传连锁图谱的构建提供了依据，也为海棠的分子育种工作奠定了基础。
Molecular markers and molecular genetic maps are prerequisites for molecular breeding in any plant species. A comprehensive genetic linkage map for cultivated Porphyra haitanensis T. J. Chang et B. F. Zheng has not yet been developed. In this study, 157 double haploid (DH) lines [derived from a YSIII (wildtype) × RTPM (red‐type artificial pigmentation mutant) cross] were used as a mapping population in P. haitanensis. A total of 60 pairs of sequence‐related amplified polymorphism (SRAP) primers and 39 pairs of simple sequence repeat (SSR) primers were used to detect polymorphisms between the two parents. Fifteen SRAP and 16 SSR polymorphic primer pairs were selected to analyze the DH population. A linkage genetic map comprising 67 SRAP markers and 20 SSR markers in five linkage groups, with a total length of 830.6 cM and an average of 10.13 cM between markers, was constructed. The markers were distributed evenly in all linkage groups without clustering. The linkage groups comprised 12–23 markers ranging in length from 134.2 to 197.3 cM. The estimated genome length of P. haitanensis was 942.4 cM, with 88.1% coverage. This is the first report of a comprehensive genetic map in P. haitanensis. The map presented here will provide a basis for the development of high‐density genetic linkage maps and lay the foundation for molecular breeding work in P. haitanensis.