CD11c- and CD11b-expressing mouse leukocytes transport single Toxoplasma gondii tachyzoites to the brain
CD11c- and CD11b-expressing mouse leukocytes transport single Toxoplasma gondii tachyzoites to the brain
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DOI：
10.1182/blood-2005-02-0666
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发表时间：
2006-01-01
期刊：
影响因子：
20.3
通讯作者：
Tardieux, I
中科院分区：
文献类型：
--
作者：
Courret, N;Darche, S;Tardieux, I
原生动物寄生虫刚地弓形虫通过肠粘膜进入宿主，并在大脑等远处组织中定居，其后代在大脑中终生存在。我们研究了CD 11 c和CID 11b表达白细胞在弓形虫从小鼠小肠寄生的早期阶段和随后的寄生虫在大脑中的定位过程中的作用。在小鼠胃内接种含囊寄生虫后，来自肠固有层、淋巴结和肠系膜淋巴结的CD 11 c（+）树突状细胞被寄生，而在血液中，寄生虫与CD 11 c（-）CD 11b（+）单核细胞相关。使用过继转移实验，我们证明了这些寄生细胞在幼稚受体小鼠的大脑中引发了寄生过程。离体分析寄生的白细胞表明，单个速殖子仍然在细胞周边，往往包围宿主细胞质膜，但不分裂。使用标记循环白细胞的染料或已知阻止CD 11 b（+）循环白细胞离开微血管床腔的抗体，以及造血细胞表达绿色荧光蛋白的嵌合小鼠，我们确定弓形虫子虫劫持CD 11 b（+）白细胞到达脑血管外空间。
The protozoan parasite Toxoplasma gondii enters hosts through the intestinal mucosa and colonizes distant tissues such as the brain, where its progeny persists for a lifetime. We investigated the role of CD11c- and CID11b-expressing leukocytes in T gondii transport during the early step of parasitism from the mouse small intestine and during subsequent parasite localization in the brain. Following intragastric inoculation of cystcontaining parasites in mice, CD11c(+) dendritic cells from the intestinal lamina propria, the payer patches, and the mesenteric lymph nodes were parasitized while in the blood, parasites were associated with the CD11c(-) CD11b(+) monocytes. Using adoptive transfer experiments, we demonstrated that these parasitized cells triggered a parasitic process in the brain of naive recipient mice. Ex vivo analysis of parasitized leukocytes showed that single tachyzoites remained at the cell periphery, often surrounded by the host cell plasma membrane, but did not divide. Using either a dye that labels circulating leukocytes or an antibody known to prevent CD11b(+) circulating leukocytes from leaving the microvascular bed lumen, and chimeric mice in which the hematopoietic cells expressed the green fluorescent protein, we established that T gondii zoites hijacked CD11b(+) leukocytes to reach the brain extravascular space.