Anaerofustis stercorihominis gen. nov., sp nov., from human feces
Anaerofustis stercorihominis gen. nov., sp nov., from human feces
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DOI：
10.1016/j.anaerobe.2003.10.002
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发表时间：
2004-02-01
期刊：
影响因子：
2.3
通讯作者：
Collins, MD
中科院分区：
文献类型：
--
作者：
Finegold, SM;Lawson, PA;Collins, MD
对从人类粪便中分离出的一种未鉴定的革兰氏阳性、严格厌氧、不形成孢子的杆状细菌进行了表型和系统发育研究。该生物体呈过氧化氢酶阴性，对 20% 胆汁具有抗性，产生乙酸和丁酸作为葡萄糖代谢的终产物，并且 G + C 含量约为 70 mol%。比较16S rRNA基因测序表明，该不明细菌是革兰氏阳性菌梭菌亚门的成员，并与rRNA簇XV形成松散关联。在未鉴定的细菌和该 rRNA 簇及相关 rRNA 簇内的所有其他已识别物种之间观察到 12% 或更大的序列分歧值。树分析显示，这种未知的厌氧菌在 rRNA 簇 XV 的基部附近形成了一条深线分支，在系统发育上代表了一个迄今为止未知的分类群，与醋杆菌属、严格意义上的真杆菌属、假杆菌属和其他相关生物体不同。基于系统发育和表型证据，有人建议将粪便中的未知细菌归类为新的厌氧菌属，即 Anaerofustis stercorihominis sp。十一月粪人厌氧菌的模式菌株是 ATCC BAA-858(T) = CCUG 47767(T)。 (C) 2003 Elsevier Ltd. 保留所有权利。
Phenotypic and phylogenetic studies were performed on an unidentified Gram-positive, strictly anaerobic, non-spore-forming, rod-shaped bacterium isolated from human feces. The organism was catalase-negative, resistant to 20% bile, produced acetic and butyric acids as end products of glucose metabolism, and possessed a G + C content of approximately 70 mol %. Comparative 16S rRNA gene sequencing demonstrated that the unidentified bacterium was a member of the Clostridium sub-phylum of the Gram-positive bacteria, and formed a loose association with rRNA cluster XV. Sequence divergence values of 12% or greater were observed between the unidentified bacterium and all other recognized species within this and related rRNA clusters. Treeing analysis showed the unknown anaerobe formed a deep line branching near to the base of rRNA cluster XV and phylogenetically represents a hitherto unknown taxon, distinct from Acetobacterium, Eubacterium sensu stricto, Pseudoramibacter and other related organisms. Based on both phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium from feces be classified in a new genus Anaerofustis, as Anaerofustis stercorihominis sp. nov. The type strain of Anaerofustis stercorihominis is ATCC BAA-858(T) = CCUG 47767(T). (C) 2003 Elsevier Ltd. All rights reserved.