Alkaline, acid, and neutral phosphatase activities are induced during development in Myxococcus xanthus.
Alkaline, acid, and neutral phosphatase activities are induced during development in Myxococcus xanthus.
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黄色粘球菌发育过程中会诱导碱性、酸性和中性磷酸酶活性。
DOI：
10.1128/jb.172.5.2294-2302.1990
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发表时间：
1990
影响因子：
3.2
通讯作者：
Zusman,DR
中科院分区：
文献类型：
--
作者：
Weinberg,RA;Zusman,DR
据报道，刺激黄色粘球菌子实体形成的重要信号之一是磷酸盐饥饿。因此，我们选择研究M. xanthus发育许多磷酸酶可以切割底物对硝基苯酚磷酸。在不同pH值的缓冲液中使用该底物，我们获得了M. xanthus结果表明，M. xanthus：两个营养和三个发育。这两种独特的营养活性的最适pH值为7.2和8.5。两者都需要镁，并且都被还原剂二硫苏糖醇抑制。发育（孢子）活动模式的最适pH值为5.2，7.2和8.5。所有这三项活动都是独立于Mg的。二硫苏糖醇仅抑制碱性磷酸酶活性。酸性磷酸酶的活性在发育的早期，在最初的2 - 4小时内被诱导。中性和碱性磷酸酶Mg-独立的活动诱导晚得多，约粘孢子变得明显的时间（24至30小时）。这三种活性在发芽后大大减少;然而，损失的动力学对于所有三种都不同。酸性磷酸酶活性下降得非常快，中性磷酸酶活性仅在孢子开始转化为杆状体后才开始下降，碱性磷酸酶活性一直保持高水平，直到细胞开始分裂。所有三个发育活动进行了测量的发育信号突变体携带asg，csg和dsg。在突变体中获得的表达模式与在发育过程中表现出类似表达模式的其他发育调控基因的表达模式一致。容易与磷酸酶可以进行测定，应使本报告中所述的活动有用的生化标志物的阶段的子实体的形成和萌发。
One of the signals that has been reported to be important in stimulating fruiting body formation of Myxococcus xanthus is starvation for phosphate. We therefore chose to study phosphatase activity during M. xanthus development. Many phosphatases can cleave the substrate p-nitrophenol phosphate. Using this substrate in buffers at various pHs, we obtained a profile of phosphatase activities during development and germination of M. xanthus. These experiments indicated that there are five patterns of phosphatase activity in M. xanthus: two vegetative and three developmental. The two uniquely vegetative activities have pH optima at 7.2 and 8.5. Both require magnesium and both are inhibited by the reducing agent dithiothreitol. The developmental (spores) patterns of activity have pH optima of 5.2, 7.2, and 8.5. All three activities are Mg independent. Only the alkaline phosphatase activity is inhibited by dithiothreitol. The acid phosphatase activity is induced very early in development, within the first 2 to 4 h. Both the neutral and alkaline phosphatase Mg-independent activities are induced much later, about the time that myxospores become evident (24 to 30 h). The three activities are greatly diminished upon germination; however, the kinetics of loss differ for all three. The acid phosphatase activity declines very rapidly, the neutral activity begins to decline only after spores begin to convert to rods, and the alkaline phosphatase activity remains high until the time the cells begin to divide. All three developmental activities were measured in the developmental signalling mutants carrying asg, csg, and dsg. The pattern of expression obtained in the mutants was consistent with that of other developmentally regulated genes which exhibit similar patterns of expression during development. The ease with which phosphatases can be assayed should make the activities described in this report useful biochemical markers of stages of both fruiting body formation and germination.