Immunological study of the glial fibrillary acidic protein.
Immunological study of the glial fibrillary acidic protein.
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胶质纤维酸性蛋白的免疫学研究。
DOI：
10.1016/0006-8993(72)90347-2
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发表时间：
1972
期刊：
影响因子：
2.9
通讯作者：
A. Bignami
中科院分区：
文献类型：
--
作者：
C. Uyeda;C. Uyeda;L. Eng;L. Eng;A. Bignami
文献中报道了许多脑蛋白的免疫学研究。已生产出针对全脑水蛋白提取物的抗体S, 7, 14；对抗 S-100 蛋白，一种仅限于神经系统的酸性蛋白；以及针对纯化的结构蛋白：髓磷脂 6 的碱性蛋白和微管蛋白 12, 13。最近报道了作为具有严重纤维神经胶质增生的人脑组织的主要成分的酸性蛋白的分离4。在本文中，将描述针对神经胶质纤维酸性（GFA）人类蛋白的抗体的制备。结果表明，正常人脑和星形细胞瘤与抗GFA抗体发生交叉反应，与周围神经或其他组织和器官不发生交叉反应。此外，还将报告通过十二烷基硫酸钠 (SDS) 中的聚丙烯酰胺凝胶电泳估算的 GFA 蛋白的分子量。
Numerous immunological studies of brain proteins have been reported in the literature. Antibodies have been produced against aqueous protein extracts of whole brainS, 7, 14; against the S-100 protein, an acidic protein restricted to the nervous systemS; and against purified structural proteins: the basic protein of myelin 6 and the microtubular protein12, 13.The isolation of an acidic protein as a major component of human brain tissue with severe fibrillary gliosis has been recently reported 4. In this paper the preparation of antibodies against the glial fibrillary acidic (GFA) human protein will be described. It will be shown that normal human brain and astrocytoma cross-react with anti-GFA antibodies, and that no cross reaction occurs with peripheral nerve or with other tissues and organs. In addition, the molecular weight of the GFA protein as estimated by polyacrylamide gel electrophoresis in sodium dodecyl sulfate (SDS) will be reported.