Expression, purification, crystallization and preliminary X-ray analysis of human spindlin1, an ovarian cancer-related protein
Expression, purification, crystallization and preliminary X-ray analysis of human spindlin1, an ovarian cancer-related protein
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DOI：
10.2174/092986606775101661
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发表时间：
2006-01-01
影响因子：
1.6
通讯作者：
Rao, ZH
中科院分区：
文献类型：
--
作者：
Jiang, FG;Zhao, QA;Rao, ZH
人spindlin I是新筛选鉴定的与卵巢癌相关的基因产物，与小鼠spindlin高度同源。它是在小鼠从卵母细胞到胚胎的转变过程中表达的丰富的母体转录物。在此，重组人 Spindlin I 已在大肠杆菌 BL21 中过表达，并使用悬滴蒸气扩散法进行纯化和结晶。使用硫酸铵作为沉淀剂获得衍射分辨率为 2.25 埃的晶体。该晶体属于空间群 P2(1)2(1)2(1)，晶胞参数 a = 40.7 埃，b = 84.4 埃，c = 136.4 埃，α = beta = gamma = 90 度。假设每个不对称单元有两个分子，则溶剂含量计算为 42.4%。
Human spindlin I is a newly screened and identified gene product related to ovarian carcinomas and is highly homologous to mouse spindlin. It is an abundant maternal transcript expressed in the mouse during the transition from oocyte to embryo. Here, the recombinant human spindlin I has been overexpressed in Escherichia coli BL21, purified and crystallized using the hanging-drop vapour-diffusion method. Crystals diffracting to 2.25 angstrom resolution were obtained using ammonium sulfate as precipitant. The crystals belong to the space group P2(1)2(1)2(1), with unit-cell parameters a = 40.7 angstrom, b = 84.4 angstrom, c =136.4 angstrom, alpha = beta = gamma = 90 degrees. Assuming two molecules per asymmetric unit, the solvent content is calculated to be 42.4%.