Epigallocatechin gallate inhibits growth and epithelial-to-mesenchymal transition in human thyroid carcinoma cell lines
Epigallocatechin gallate inhibits growth and epithelial-to-mesenchymal transition in human thyroid carcinoma cell lines
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DOI：
10.1002/jcp.24372
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发表时间：
2013-10-01
影响因子：
5.6
通讯作者：
Ando, Sebastiano
中科院分区：
文献类型：
--
作者：
De Amicis, Francesca;Perri, Anna;Ando, Sebastiano
分化良好的甲状腺乳头状癌和滤泡性甲状腺癌是最常见的甲状腺癌类型，预后一般良好，但也有一些患者复发。表没食子儿茶素-3-没食子酸酯（EGCG）是绿茶中的一种主要儿茶素，已被证明对各种类型的人类癌症具有显着的治疗潜力，尽管关于甲状腺癌细胞的数据仍然缺乏。本研究旨在探讨EGCG对人甲状腺乳头状（FB-2）和滤泡（WRO）癌细胞增殖和运动的影响。我们的研究结果表明，EGCG（10、40、60M）处理抑制FB-2和WRO细胞的生长呈剂量依赖性。这些变化与细胞周期蛋白D1减少、p21和p53表达增加有关。此外，EGCG抑制AKT和ERK1/2的磷酸化。此外，EGCG处理导致细胞运动和迁移的减少。FB-2的运动和迁移变化与参与细胞粘附和肌动蛋白细胞骨架重组的几种蛋白质表达的调节有关。24h后，EGCG引起E-cadherin表达升高，同时SNAIL、ZEB和基本螺旋-环-螺旋转录因子TWIST表达降低。除Vimentin表达下调外，N-cadherin和5-整合素表达下调。这些数据与明胶酶谱法证明的MMP9活性降低有很好的相关性。我们的研究结果支持EGCG对甲状腺癌细胞增殖和运动的抑制作用，同时伴有上皮细胞向间充质细胞转化标记物的缺失。j .细胞。中国生物医学工程学报，2011,31(2):444 - 444。(c) 2013 Wiley Periodicals, Inc.；
Well-differentiated papillary and follicular thyroid carcinoma are the most frequent types of thyroid cancer and the prognosis is generally favorable however, a number of patients develops recurrences. Epigallocatechin-3-gallate (EGCG), a major catechin in green tea, was shown to possess remarkable therapeutic potential against various types of human cancers, although data on thyroid cancer cells are still lacking. The aim of this study was to investigate the effect of EGCG on the proliferation and motility of human thyroid papillary (FB-2) and follicular (WRO) carcinoma cell lines. Our results demonstrate that EGCG (10, 40, 60M) treatment inhibited the growth of FB-2 and WRO cells in a dose-dependent manner. These changes were associated with reduced cyclin D1, increased p21 and p53 expression. Furthermore, EGCG suppressed phosphorylation of AKT and ERK1/2. In addition EGCG treatment results in reduction of cell motility and migration. Changes in motility and migration in FB-2 were associated with modulation in the expression of several proteins involved in cell adhesion and reorganization of actin cytoskeleton. After 24h EGCG caused an increase of the E-cadherin expression and a concomitant decrease of SNAIL, ZEB and the basic helix-loop-helix transcription factor TWIST. Besides expression of Vimentin, N-cadherin and 5-integrin was down-regulated. These data well correlate with a reduction of MMP9 activity as evidenced by gelatin zymography. Our findings support the inhibitory role of EGCG on thyroid cancer cell proliferation and motility with concomitant loss of epithelial-to-mesenchymal cell transition markers. J. Cell. Physiol. 228: 2054-2062, 2013. (c) 2013 Wiley Periodicals, Inc.