Characterization of multipotent adult stem cells from the skin: transforming growth factor-beta (TGF-beta) facilitates cell growth.
Characterization of multipotent adult stem cells from the skin: transforming growth factor-beta (TGF-beta) facilitates cell growth.
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DOI：
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发表时间：
2004
影响因子：
3.7
通讯作者：
Y. Kawase;Y. Yanagi;T. Takato;M. Fujimoto;H. Okochi
中科院分区：
文献类型：
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作者：
Y. Kawase;Y. Yanagi;T. Takato;M. Fujimoto;H. Okochi
最近，成体干细胞被从皮肤中分离出来，并被指定为皮肤来源的前体细胞(SKPs)。这些SKP在体外培养后，可以分化为神经元、神经胶质细胞、平滑肌细胞和脂肪细胞。在目前的研究中，我们使用球状培养系统在含有甲基纤维素的培养基中证实了SKP的克隆性扩张。在众多的生长因子中，只有转化生长因子-β(TGF-β)与表皮生长因子和碱性成纤维细胞生长因子联合使用，才能单独促进SKPs的球体形成和增殖。此外，在球体形成条件下，转化生长因子-β不改变SKP的表型特征。在神经干细胞中未观察到转化生长因子-β对球体形成的影响，神经干细胞表达一组与SKP不同的细胞表面标记，表明SKP具有不同的特征。尽管SKP的数量随着年龄的增长而减少，但在所有年龄段，转化生长因子-β都促进了球状集落的形成和增殖。这些结果表明，在培养过程中保持转化生长因子-β的SKP在使用成人组织来源的细胞替代治疗中具有潜在的用途。
Recently, adult stem cells have been isolated from the skin and designated as skin-derived precursors (SKPs). These SKPs, cultured in vitro, can give rise to neurons, glia, smooth muscle cells, and adipocytes. In the current study, we confirmed the clonal expansion of SKPs using a sphere-forming culture system in a medium containing methylcellulose. Among the growth factors, only transforming growth factor-beta (TGF-beta) was revealed to uniquely facilitate the sphere formation and proliferation of the SKPs in combination with EGF and bFGF. In addition, TGF-beta did not alter phenotypical characteristics of the SKPs under sphere-forming conditions. The effect of TGF-beta on sphere formation was not observed in neural stem cells, which expressed a different set of cell surface markers from SKPs, suggesting that SKPs have distinct features. Although the number of SKPs decreased with age, TGF-beta increased the sphere colony formation and proliferation in all ages. These results suggest that SKPs maintained in the presence of TGF-beta during culture are of potential use in cell-replacement therapies employing adult tissue sources.