Stable-hydrogen isotope heterogeneity in keratinous materials: mass spectrometry and migratory wildlife tissue subsampling strategies.
Stable-hydrogen isotope heterogeneity in keratinous materials: mass spectrometry and migratory wildlife tissue subsampling strategies.
复制标题
角质材料中的稳定氢同位素异质性：质谱分析和迁徙野生动物组织二次采样策略。
DOI：
10.1002/rcm.2626
复制
发表时间：
2006
期刊：
影响因子：
--
通讯作者：
K. Hobson
中科院分区：
文献类型：
--
作者：
L. Wassenaar;K. Hobson
生物组织的稳定氢同位素测量（deltaD）在野生动物和法医学研究中获得了广泛的认可，特别是在跟踪鸟类和其他物种的地理运动方面。连续流同位素比质谱法能够对小至0.15 mg的组织样品进行高通量deltaD分析，而传统的离线分析需要7-10 mg。样本量的减少引起了人们对样本内氢同位素差异的担忧，这是由于潜在的生物异质性可能超出对地理空间来源的解释。为了帮助解决这个问题，羽毛从圈养鸟类，以检查同位素变异预期由于样本大小，位置和异质性因素，并从选定的野生鸟类检查同位素变异由于这些和额外的饮食或位置变化在羽毛生长。对捕获的鸟类羽毛沿单个羽毛两侧的叶片沿着以0.25、0.35、0.45、0.6、1.0和2.0 mg的质量进行子采样，并沿羽轴沿着进行子采样。结果表明，在广泛的样品质量范围内的羽毛deltaD测量的一致性。羽毛内δ D同位素变异的圈养和一些野生鸟类是低至+/-3千分之一的叶片材料，这相当于一个地理空间分辨率约1度的纬度在北美中部。轴的样本内方差为+/-5 ‰，野生和圈养鸟类的deltaD值较低。然而，考虑到在一些野生物种中观察到的非凡的羽毛内δ D变异，我们建议研究人员首先仔细评估感兴趣的物种在选定的组织生长期内样本内和样本间氢同位素变异的程度，然后再尝试对起源进行地理空间解释。
Stable-hydrogen isotope measurements (deltaD) of biological tissues have gained widespread acceptance in wildlife and forensic studies, especially in tracking geographical movements of birds and other species. Continuous-flow isotope-ratio mass spectrometry enables high-throughput deltaD analyses to be conducted on tissue samples as small as 0.15 mg, compared with conventional offline analyses that require 7-10 mg. This reduction in sample size has raised concerns regarding intra-sample hydrogen isotopic variance due to potential biological heterogeneities that could exceed interpretations of geospatial origin. To help resolve this, feathers were obtained from captive birds to examine isotopic variance expected due to sample size, location, and heterogeneity factors, and from selected wild birds to examine isotopic variance due to these and to additional dietary or location changes during feather growth. Captive bird feathers were sub-sampled along the vane on either side of a single feather at masses of 0.25, 0.35, 0.45, 0.6, 1.0 and 2.0 mg, and along the rachis. The results showed consistency of feather deltaD measurements across a wide range of sample masses. Within-feather deltaD isotopic variance for captive and some wild birds was as low as +/-3 per thousand for vane material, which corresponds to a geospatial resolution of about 1 degree of latitude in central North America. Intra-sample variance for the rachis was +/-5 per thousand, with lower deltaD values for both wild and captive birds. However, given the extraordinary intra-feather deltaD variance observed in some wild species, we recommend researchers first carefully assess the degree of intra- and inter-sample hydrogen isotopic variation in the selected tissue growth period for the species of interest before geospatial interpretations of origin are attempted.