Differential hypersensitivity to DNase I in the regulatory region of human hsp90β gene in heat shock and constitutive expression
Differential hypersensitivity to DNase I in the regulatory region of human hsp90β gene in heat shock and constitutive expression
复制标题
DOI：
10.1016/s1357-2725(02)00197-8
复制
发表时间：
2003-03-01
影响因子：
4
通讯作者：
Shen, YF
中科院分区：
文献类型：
--
作者：
Liu, BS;Wang, N;Shen, YF
DNA酶I超敏反应分析是研究染色质结构变化对基因表达影响的有用工具。为了阐明染色质对人hsp 90 β基因的调控，研究了未经处理和热休克的Jurkat细胞对DNA酶I的不同敏感性。在hsp 90 β基因转录起始位点上，发现了4个主要的超敏位点，分别位于-120/-20bp（HS 1）、+360bp（HS 2）、+630/+780bp（HS 4）和+1020bp（HS 5）附近。HS 1由组成型和热休克共享，而HS 4和HS 5的内含子位点由热休克引起，HS 2仅在组成型表达中示出。此外，在组成型表达和热休克表达中都发现了上游约8.7 kb和下游约6.8 kb的远端HS，这表明hsp 90 β基因的边界延伸了约16 kb。HS图谱证实了在不同处理下hsp 90 β基因表达的染色质调控。(C)2002爱思唯尔科技有限公司版权所有。
DNase I hypersensitivity analysis is a useful tool to investigate impact of structure changes in chromatin on the expression of a gene. In order to unravel chromatin regulation on human hsp90beta gene, differential sensitivity to DNase I in non-treated and heat-shocked Jurkat cells are examined. Four major hypersensitive sites at -120/-20bp (HS1), +360bp (HS2), +630/+780 by (HS4) and around +1020 by (HS5) with respect to the transcription start site of hsp90beta gene have been identified. The HS1 is shared by both constitutive and heat shock, while the intronic sites of HS4 and HS5 are elicited by heat shock and HS2 is illustrated only in constitutive expression. In addition, distal HSs at around 8.7 kb upstream and 6.8 kb downstream are found in both constitutive and heat shock expression, which indicate that the boundaries of the hsp90beta gene extend for some 16 kb. The HS patterns confirm chromatin regulation in the expression of hsp90beta gene under various treatments. (C) 2002 Elsevier Science Ltd. All rights reserved.