Soybean glycinin and β-conglycinin damage the intestinal barrier by triggering oxidative stress and inflammatory response in weaned piglets
Soybean glycinin and β-conglycinin damage the intestinal barrier by triggering oxidative stress and inflammatory response in weaned piglets
复制标题
DOI：
10.1007/s00394-023-03188-8
复制
发表时间：
2023-06-26
影响因子：
5
通讯作者：
Wang，Xichun
中科院分区：
文献类型：
--
作者：
Wang，Lei;Li，Wen;Wang，Xichun
大豆球蛋白(β-congicinin，7S)和大豆球蛋白(11S)是大豆中的主要抗原性蛋白，在幼年动物体内可引起多种变态反应。本研究旨在探讨7S和11S变应原对仔猪肠道的影响。方法将30头21日龄健康断奶的“杜洛克 × 长白 × 约克夏”仔猪随机分为基础日粮组、7S组和11S组，分别饲喂基础日粮、7S组和11S组。检测过敏标志物、肠道通透性、氧化应激和炎症反应，并观察肠道组织的不同切片。免疫组织化学、RT-qPCR和WB法检测NLRP-3信号通路相关基因和蛋白的表达。典型的过敏标志物包括IgE的产生和组胺和5-羟色胺(5-HT)的显著升高。在实验性断奶仔猪中，观察到更具侵袭性的肠道炎症和屏障功能障碍。此外，补充7S和11S会增加8-羟基-2脱氧鸟苷(8-OHdG)和硝基酪氨酸的水平，从而触发氧化应激。此外，在断奶仔猪的十二指肠、空肠和回肠组织中，NLRP-3、炎症体ASC、半胱氨酸天冬氨酸氨基转移酶-1、IL-1β和IL-18的表达水平较高。结论7S和11S损伤断奶仔猪的肠道屏障，可能与氧化应激和炎症反应的发生有关。然而，这些反应背后的分子机制值得进一步研究。
PurposeSoybean glycinin (11S) andβ-conglycinin (7S) are major antigenic proteins in soybean and can induce a variety of allergic reactions in the young animals. This study aimed to investigate the effect of 7S and 11S allergens on the intestine of piglets.MethodsThirty healthy 21-day-old weaned “Duroc × Long White × Yorkshire” piglets were randomly divided into three groups fed with the basic diet, the 7S supplemented basic diet, or the 11S supplemented basic diet for 1 week. Allergy markers, intestinal permeability, oxidative stress, and inflammatory reactions were detected, and we observed different sections of intestinal tissue. The expressions of genes and proteins related to NOD-like receptor thermal protein domain associated protein 3 (NLRP-3) signaling pathway were detected by IHC, RT-qPCR, and WB.ResultsSevere diarrhea and decreased growth rate were observed in the 7S and 11S groups. Typical allergy markers include IgE production and significant elevations of histamine and 5-hydroxytryptamine (5-HT). More aggressive intestinal inflammation and barrier dysfunction were observed in the experimental weaned piglets. In addition, 7S and 11S supplementation increased the levels of 8-hydroxy-2 deoxyguanosine (8-OHdG) and nitrotyrosine, triggering oxidative stress. Furthermore, higher expression levels of NLRP-3 inflammasome ASC, caspase-1, IL-1β, and IL-18 were observed in the duodenum, jejunum, and ileum.ConclusionWe confirmed that 7S and 11S damaged the intestinal barrier of weaned piglets and may be associated with the onset of oxidative stress and inflammatory response. However, the molecular mechanism underlying these reactions deserves further study.