Simultaneous Transcriptome Analysis of Host and Pathogen Highlights the Interaction Between Brassica oleracea and Sclerotinia sclerotiorum
Simultaneous Transcriptome Analysis of Host and Pathogen Highlights the Interaction Between Brassica oleracea and Sclerotinia sclerotiorum
复制标题
宿主和病原体的同步转录组分析强调了甘蓝和核盘菌之间的相互作用
DOI：
10.1094/phyto-06-18-0204-r
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发表时间：
2019-04-01
期刊：
影响因子：
3.2
通讯作者：
Qian, Wei
中科院分区：
文献类型：
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作者：
Ding, Yijuan;Mei, Jiaqin;Qian, Wei
白霉病是由菌核菌引起的一种严重危害芸苔属作物的病害。在这里，我们同时评估了菌丝体在抗病(R)和易感(S)甘蓝菌群中产生的病变的转录组变化，这些菌群来自抗性分离的F2群体。接种后12 h，菌核病菌毒力基因（包括聚半乳糖醛酸、几丁质合成酶、分泌蛋白和草酸生物合成）在甘蓝芽孢杆菌病灶中被显著抑制，但在接种后24 h，菌核病菌在甘蓝芽孢杆菌病灶中的表达模式相似。在0 ~ 12 hpi期间，耐药的甘蓝诱导了可能感知菌核信号的受体的表达，并采取了复杂的策略来抑制病原菌的建立，包括通过激活Ca2+信号快速积累活性氧和抑制病原菌草酸的产生。此外，耐药甘蓝细胞壁降解被抑制，可能阻止菌核菌菌丝的扩大。菌核葡萄球菌和寄主的转录组变化表明，耐药甘蓝在早期感染时对菌核葡萄球菌产生强烈的应答。
White mold disease caused by Sclerotinia sclerotiorum is a devastating disease of Brassica crops. Here, we simultaneously assessed the transcriptome changes from lesions produced by S. sclerotiorum on disease-resistant (R) and -susceptible (S) B. oleracea pools bulked from a resistance-segregating F2 population. Virulence genes of S. sclerotiorum, including polygalacturonans, chitin synthase, secretory proteins, and oxalic acid biosynthesis, were significantly repressed in lesions of R B. oleracea at 12 h postinoculation (hpi) but exhibited similar expression patterns in R and S B. oleracea at 24 hpi. Resistant B. oleracea induced expression of receptors potentially to perceive Sclerotinia signals during 0 to 12 hpi and deployed complex strategies to suppress the pathogen establishment, including the quick accumulation of reactive oxygen species via activating Ca2+ signaling and suppressing pathogen oxalic acid generation in S. sclerotiorum. In addition, cell wall degradation was inhibited in the resistant B. oleracea potentially to prevent the expansion of Sclerotinia hyphae. The transcriptome changes in S. sclerotiorum and host revealed that resistant B. oleracea produces strong responses against S. sclerotiorum during early infection.