Involvement of iclR and rpoS in the induction of acs, the gene for acetyl coenzyme A synthetase of Escherichia coli K-12.
Involvement of iclR and rpoS in the induction of acs, the gene for acetyl coenzyme A synthetase of Escherichia coli K-12.
复制标题
DOI：
10.1111/j.1574-6968.1997.tb10178.x
复制
发表时间：
1997
影响因子：
2.1
通讯作者：
S. Shin;S. Song;D. S. Lee;J. Pan;C. Park
中科院分区：
文献类型：
--
作者：
S. Shin;S. Song;D. S. Lee;J. Pan;C. Park
大肠杆菌中有两条独立的途径将乙酸转化为乙酰辅酶A：磷酸转乙酰酶和乙酸激酶逆转乙酸的产生，以及清除乙酸的乙酰辅酶A合成酶（Acs）途径。我们研究了acs基因的表达，通过使用猫转录融合。据观察，acs表达根据所使用的碳源而变化，并且即使在不存在乙酸盐的情况下也发生在生长的稳定期。乙醛酸分流的阻遏物iclR和固定相σ因子rpoS中的突变减少了Acs介导的乙酸盐的消耗，表明两者都参与了acs调节。
Two independent pathways in Escherichia coli convert acetate to acetyl CoA: reversal of acetate production by phosphotransacetylase and acetate kinase, and the acetyl-CoA synthetase (Acs) pathway that scavenges acetate. We investigated acs gene expression by using a cat transcriptional fusion. It was observed that acs expression varies depending on the carbon sources used and occurs in the stationary phase of growth even in the absence of acetate. Mutations in iclR for the repressor of the glyoxylate shunt and in rpoS for the stationary phase sigma factor reduced the consumption of acetate mediated by Acs, indicating that both are involved in acs regulation.