Immunosuppressive properties of a peptic fragment of BSA.
Immunosuppressive properties of a peptic fragment of BSA.
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BSA 消化片段的免疫抑制特性。
DOI：
10.4049/jimmunol.119.4.1340
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发表时间：
1977
影响因子：
4.4
通讯作者：
J. Michael
中科院分区：
文献类型：
--
作者：
A. Muckerheide;A. Pesce;J. Michael
研究了牛血清白蛋白(BSA)一段消化片段的免疫原性。BSA用胃酶进行有限的蛋白质降解，得到的片段用DEAE纤维素层析分离。考虑中的碎片，组分Ia(M.W.8000到10,000)，不与抗BSA血清沉淀，但抑制了特异性抗体与标记的BSA的结合，表明存在天然抗原上的决定簇。用A1(OH)3凝胶或完全弗氏佐剂中的组分Ia免疫BDF1小鼠，经被动皮肤过敏试验(PCA)或改良的Farr法检测，均未产生明显的抗体应答。该片段在抗BSA血清致敏的小鼠皮肤上引起PCA反应。在BSA免疫前不同时间给小鼠单次注射组分Ia，可显著抑制抗BSA抗体的形成。更详细地研究了胃酸片段抑制IgE反应的条件。有证据表明，这种抑制可以归因于我们的系统中存在特定的T抑制细胞。
The immunogenic properties of a peptic fragment of BSA were investigated. BSA was subjected to limited proteolysis by pepsin and the resulting fragments were separated on DEAE cellulose. The fragment under consideration, Fraction Ia (m.w. 8000 to 10,000), did not precipitate with anti-BSA serum but did inhibi, the binding of specific antibody to labeled BSA, indicating the presence of determinants found on the native antigen. BDF1 mice immunized with Fraction Ia in A1 (OH)3 gel or in complete Freund's adjuvant produced no significant antibody response as measured by passive cutaneous anaphylited a (PCA) or by a modified Farr assay. The fragment elicited a PCA reaction in mouse skin sensitized with anti-BSA serum. Treatment of mice with single doses of Fraction Ia at various time intervals before immunization with BSA resulted in significant suppression of the formation of anti-BSA antibody. The conditions of suppresion of the IgE response by the peptic fragment were studied in greater detail. Evidence is presented that such suppression can be attributed to the presence of specific T suppressor cells in our system.