Large-scale purification of latex bead phagosomes from mouse macrophage cell lines and subsequent preparation for high-throughput quantitative proteomics.
Large-scale purification of latex bead phagosomes from mouse macrophage cell lines and subsequent preparation for high-throughput quantitative proteomics.
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DOI：
10.1007/978-1-59745-157-4_22
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发表时间：
2008
影响因子：
--
通讯作者：
A. Rupper;J. Cardelli
中科院分区：
文献类型：
--
作者：
A. Rupper;J. Cardelli
吞噬作用涉及多种细胞表面受体的参与，这些受体的信号必须整合在形成吞噬体杯的膜上。该方法能够定量蛋白质组学分析的吞噬体馏分来源于两种不同条件下刺激的吞噬细胞，从而允许吞噬体信号的复杂性进行分析的吞噬体馏分蛋白质含量的定量变化。
Phagocytosis involves the engagement of a diverse array of cell surface receptors whose signals must be integrated on the membrane of the forming phagosomal cup. This method enables the quantitative proteomic analysis of phagosome fractions derived from phagocytes stimulated under two different conditions, thus allowing the complexity of phagosomal signaling to be analyzed in terms of the quantitative changes in phagosomal fraction protein content.