Monoclonal Antibody Preparation and Expression Profile Analysis of a Novel Hepatoma Associated Gene
Monoclonal Antibody Preparation and Expression Profile Analysis of a Novel Hepatoma Associated Gene
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DOI：
10.1007/s12253-013-9703-4
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发表时间：
2014-04
影响因子：
2.8
通讯作者：
Yan-hong Liu;Jie Song;Yuehui Li;Yanjie Zhao;Q. Ju;Guo-hua Zhou;Guancheng Li
中科院分区：
文献类型：
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作者：
Yan-hong Liu;Jie Song;Yuehui Li;Yanjie Zhao;Q. Ju;Guo-hua Zhou;Guancheng Li
肝癌相关基因（Hepatoma associated gene，HTA）是我们前期筛选克隆的一个基因，在某些肿瘤中特异性表达，在肝细胞癌（Hepatocellular carcinoma，HCC）中具有促癌作用。为进一步阐明HTA在肝癌发生中的作用机制及其作为肿瘤生物标志物的潜在价值，本研究利用原核重组表达系统和金属亲和层析技术获得了复性HTA蛋白（HTA）。然后采用杂交瘤技术制备抗HTA单克隆抗体（mAb）。利用获得的高效价、高特异性的抗HTA mAb，通过免疫组织化学染色分析HTA的表达谱。结果表明，HTA在某些肿瘤组织中特异性表达，而在正常组织中几乎不表达。肝细胞癌组织中HTA的阳性表达率和表达量明显高于肝硬化组织、肝纤维化组织和正常肝组织。HTA的表达与肝癌的癌变过程呈正相关。
Hepatoma associated gene (HTA), a gene screened and cloned by our previous research, was specifically expressed in certain kinds of tumors and had a cancer-promoting role in hepatocellular carcinoma (HCC). To further elucidate the mechanism ofHTAin hepatoma carcinogenesis and its potential role as a cancer biomarker, refolded HTA protein (HTA) was obtained by prokaryotic recombinant expression system and immobilized metal affinity chromatography. Then anti-HTA monoclonal antibody (mAb) was produced by hybridoma technique. Using the high titer anti-HTA mAb with high specificity obtained, the expression profile of HTA was analysed by immunohistochemistry staining. It showed that HTA expressed specifically in some kinds of tumors, and didn’t express in almost any of the normal tissues. The positive expression rate and expression quantity of HTA was significantly higher in HCC tissues than in hepatic cirrhosis tissues, hepatic fibrosis tissues and normal hepatic tissues. The expression of HTA was positively correlated with hepatoma carcinogenic process.