Apolipoprotein E is synthesized in the retina by Muller glial cells, secreted into the vitreous, and rapidly transported into the optic nerve by retinal ganglion cells
Apolipoprotein E is synthesized in the retina by Muller glial cells, secreted into the vitreous, and rapidly transported into the optic nerve by retinal ganglion cells
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DOI：
10.1074/jbc.271.10.5628
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发表时间：
1996-03-08
影响因子：
4.8
通讯作者：
Fine, RE
中科院分区：
文献类型：
--
作者：
Amaratunga, A;Abraham, CR;Fine, RE
我们研究了 apoE（中枢神经系统的主要载脂蛋白）在活体兔子视网膜中的合成和运输。将 [S-35] 蛋氨酸/半胱氨酸注射到玻璃体中 4 小时后，44% 的 [S-35]Met/Cys 标记的 apoE 位于可溶性和膜封闭的视网膜部分中，而 50% 位于玻璃体中。大量完整的 [S-35]Met/Cys 标记的 apoE 在 3-6 小时内在两个可区分的囊泡区室中快速转运到视神经及其位于外侧膝状体和上丘的末端。细胞培养中的米勒胶质细胞也合成和分泌apoE。综上所述，这些结果表明 apoE 由 Muller 胶质细胞合成并分泌到玻璃体中。 ApoE 也被视网膜神经节细胞内化和/或由这些细胞合成，并作为完整分子快速转运到视神经和大脑中。我们讨论视网膜 apoE 在神经元动力学中的可能作用。
We have investigated the synthesis and transport of apoE, the major apolipoprotein of the central nervous system, in the retina of the living rabbit. Four hours after the injection of [S-35]methionine/cysteine into the vitreous, 44% of [S-35]Met/Cys-labeled apoE is in soluble and membrane-enclosed retinal fractions, while 50% is in the vitreous. A significant amount of intact [S-35]Met/Cys-labeled apoE is rapidly transported into the optic nerve and its terminals in the lateral geniculate and superior colliculus within 3-6 h in two distinguishable vesicular compartments. Muller glia in cell culture also synthesize and secrete apoE. Taken together, these results suggest that apoE is synthesized by Muller glia and secreted into the vitreous. ApoE is also internalized by retinal ganglion cells and/or synthesized by these cells and rapidly transported into the optic nerve and brain as an intact molecule. We discuss the possible roles of retinal apoE in neuronal dynamics.