HOW ACCURATELY CAN A SINGLE MOLECULE BE LOCALIZED WHEN IMAGED THROUGH AN OPTICAL MICROSCOPE?
HOW ACCURATELY CAN A SINGLE MOLECULE BE LOCALIZED WHEN IMAGED THROUGH AN OPTICAL MICROSCOPE?
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通过光学显微镜成像时，单个分子的定位精度如何？
DOI：
10.1109/isbi.2004.1398731
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Ober,RaimundJ
中科院分区：
文献类型：
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作者：
Ram,Sripad;Ward,ESally;Ober,RaimundJ
我们提出了一个简单的解析表达式，用于确定通过光学显微镜成像的单个分子的位置的精度的基本限制。该表达式取决于显微镜的光学特性和单分子的光物理特性。我们还展示了检测器像素化和检测系统中的噪声源等因素如何恶化基本限制。目前的结果使实验者能够深入了解光学显微镜可以实现的目标，并为实验设计提供指导。
We present a simple analytical expression for the fundamental limit to the accuracy with which the location of a single molecule can be determined that is imaged through an optical microscope. This expression depends on the optical properties of the microscope and the photophysical properties of the single molecule. We also show how the fundamental limit is deteriorated by factors like pixelation of the detector and noise sources in the detection system. The present results gives an experimenter insight into what is achievable in an optical microscope and provide guidelines for experimental design.