CLONING AND EXPRESSION OF STREPTOCOCCAL PYROGENIC EXOTOXIN-A AND STAPHYLOCOCCAL TOXIC SHOCK SYNDROME TOXIN-1 IN BACILLUS-SUBTILIS
CLONING AND EXPRESSION OF STREPTOCOCCAL PYROGENIC EXOTOXIN-A AND STAPHYLOCOCCAL TOXIC SHOCK SYNDROME TOXIN-1 IN BACILLUS-SUBTILIS
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DOI：
10.1007/bf00330426
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发表时间：
1987-06-01
期刊：
影响因子：
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通讯作者：
NOVICK, RP
中科院分区：
文献类型：
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作者：
KREISWIRTH, BN;HANDLEY, JP;NOVICK, RP
将编码A型链球菌致热外毒素（SPEA）和葡萄球菌中毒性休克综合征毒素-1（TSST-1）的基因克隆并在枯草芽孢杆菌中稳定表达。在非致病性芽孢杆菌背景下，重组speA克隆表达的SPE A比天然链球菌多32倍，类似地，携带tst的重组质粒在芽孢杆菌中表达的TSST-1比天然金黄色葡萄球菌多4倍。芽孢杆菌衍生的产物分泌到培养液中，对蛋白水解降解具有抗性，并且其生物学活性模拟天然制剂。
The genes encoding streptococcal pyrogenic exotoxin type A (SPE A) and staphylococcal toxic shock syndrome toxin-1 (TSST-1) were stably cloned and expressed in Bacillus subtilis. In the non-pathogenic Bacillus background, the recombinant speA clone expressed 32-fold more SPE A than the native streptococcus, and similarly, the recombinant plasmid harboring tst expressed 4-fold more TSST-1 in Bacillus than in the native Staphylococcus aureus. The Bacillus-derived products were secreted into the culture fluid, were resistant to proteolytic degradation and their biological activities mimicked native preparations.