Effects of inhalation anesthetics of Kainate-induced glutamate release from cerebellar granule cells.
Effects of inhalation anesthetics of Kainate-induced glutamate release from cerebellar granule cells.
复制标题
吸入麻醉剂对红藻氨酸诱导的小脑颗粒细胞谷氨酸释放的影响。
DOI：
10.1016/0024-3205(96)00102-6
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发表时间：
1996
期刊：
影响因子：
6.1
通讯作者：
Baker,RC
中科院分区：
文献类型：
--
作者：
Zhu,S;Baker,RC
吸入麻醉的作用机制可能包括抑制非NMDA兴奋性氨基酸神经传递。通过测量三种麻醉剂在临床相关浓度下对红藻氨酸诱导的小脑颗粒细胞谷氨酸释放的影响，解决了这种可能性。从8日龄SD大鼠获得小脑颗粒细胞，并在体外培养9-14天。用红藻氨酸盐或NMDA孵育90分钟后，通过HPLC测量培养基谷氨酸盐浓度。通过将细胞保持在空气/麻醉剂混合物的连续流动下引入吸入麻醉剂。所有测试的麻醉剂不影响NMDA诱导的谷氨酸释放。氟烷（1 MAC）、异氟烷（1 MAC）抑制红藻氨酸诱导的谷氨酸释放，而安氟烷（1 MAC）对红藻氨酸诱导的谷氨酸释放无影响。恩氟烷和其他检测的麻醉剂之间的差异表明，麻醉依赖于一个以上的过程，并且每个功能受到干扰的程度取决于所使用的特定麻醉剂。氟烷抑制红藻氨酸诱导的谷氨酸释放是不可逆的增加红藻氨酸浓度，表明氟烷不直接竞争红藻氨酸在其受体。
The mechanisms of inhalation anesthesia may include inhibiting non-NMDA excitatory amino acid neurotransmission. This possibility was addressed by measuring the effect of three anesthetics at clinically relevant concentrations on kainate-induced glutamate release from cerebellar granule cells. Cerebellar granule cells were obtained from 8-day-old SD rats and maintained in vitro for 9–14 days. Medium glutamate concentrations were measured by HPLC after 90 minutes incubation with kainate or NMDA. Inhalation anesthetics were introduced by holding the cells under a continuous flow of air/anesthetic mixtures. All anesthetics tested did not effect NMDA-induced glutamate release. Halothane (1 MAC), isoflurane (1 MAC) inhibited kainate-induced glutamate release from the cultured cells whereas enflurane (1 MAC) had no effect on kainate-induced glutamate release. The difference between enflurane and the other anesthetics tested suggests that anesthesia is dependent on more than one process and the extent at which each function is perturbed is dependent on the specific anesthetic used. Halothane inhibition of kainate-induced glutamate release was not reversible by increasing kainate concentration, indicating halothane does not directly compete with kainate at its receptor.