Molecular features and expression profiles of octopamine receptors in the brown planthopper, Nilaparvata lugens
Molecular features and expression profiles of octopamine receptors in the brown planthopper, Nilaparvata lugens
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褐飞虱 Nilaparvata lugens 章鱼胺受体的分子特征和表达谱
DOI：
10.1002/ps.5371
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发表时间：
2019
影响因子：
4.1
通讯作者：
Gao Cong-Fen
中科院分区：
文献类型：
--
作者：
Wu Shun-Fan;Jv Xiao-Min;Huang Jing-Mei;Gao Cong-Fen
章鱼胺是肾上腺素和去甲肾上腺素在无脊椎动物中的对应物，调节和调节昆虫的许多生理和行为过程。它通过与特定的章鱼胺受体结合来介导其作用，该受体属于G蛋白偶联受体（GPCR）超家族。章鱼胺受体基因的表达谱已被充分记录在不同发育阶段和多种组织类型的几个不同的目的昆虫。本研究从褐飞虱中克隆了4个章鱼胺受体基因，并对其进行了序列分析。推导的氨基酸序列与其他昆虫同源物具有高度的同源性，并具有特征性的GPCR结构域结构：七个跨膜结构域。这些基因在所有发育阶段和检查的组织中表达。N10A2B3和N10A3在卵期和1龄若虫期的表达量相对较高，而在其它各期和其它受体基因的表达量相对较低。所有这些受体基因在脑中的表达量均高于其他组织。结论本研究中章鱼胺受体基因的鉴定将为研究NlOARs所起的不同作用和探索化学品防治农业害虫的特异性靶位点提供基础。© 2019化学工业协会
BACKGROUNDOctopamine, the invertebrate counterpart of adrenaline and noradrenaline, regulates and modulates many physiological and behavioral processes in insects. It mediates its effects by binding to specific octopamine receptors, which belong to the superfamily of G‐protein coupled receptors (GPCRs). The expression profiles of octopamine receptor genes have been well documented in different developmental stages and multiple tissue types in several different insect orders. However, little work has addressed this issue in Hemiptera.RESULTSIn this study, we cloned four octopamine receptor genes from brown planthopper. The deduced amino acid sequences share high identity with other insect homologues and have the characteristic GPCRs domain architecture: seven transmembrane domains. These genes were expressed in all developmental stages and examined tissues. The expression of NlOA2B3 and NlOA3 was relatively higher in egg and first instar nymph stage than in other stages and other receptor genes. All of these receptor genes were more highly expressed in brain than other tissues.CONCLUSIONThe identification of octopamine receptor genes in this study will provide a foundation for investigating the diverse roles played by NlOARs and for exploring specific target sites for chemicals that control agricultural pests. © 2019 Society of Chemical Industry