TROPOMYOSIN - AMINO-ACID SEQUENCE AND COILED-COIL STRUCTURE
TROPOMYOSIN - AMINO-ACID SEQUENCE AND COILED-COIL STRUCTURE
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DOI：
10.1101/sqb.1973.037.01.040
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发表时间：
1973-01-01
期刊：
影响因子：
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通讯作者：
JURASEK, L
中科院分区：
文献类型：
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作者：
HODGES, RS;SODEK, J;JURASEK, L
如Steers等人所述，用溴化氰在22-25 ° C下处理蛋白质制剂20小时。（1965年出版）。溴化氰与蛋氨酸的摩尔比为100：1，在70~ 100 ℃的甲酸溶液中，蛋白质浓度为1~ 100%。通过用10体积的水稀释并冻干来终止反应。该处理导致甲硫氨酸损失94%，这些残基以高丝氨酸形式回收91%（表1）.
The protein preparation was treated with cyanogen bromide at 22-25~ for 20 hr as described by Steers et al.(1965). The molar ratio of cyanogen bromide to methionine was 100: l in 70~ formic acid at a protein concentration of 1~. The reaction was terminated by dilution with l0 volumes of water and lyophilization. This treatment resulted in a loss of 94~ of the methionines and a 91 recovery of these residues as homoserine (Table 1).